正交实验法优化桦褐孔菌多酚类物质的提取工艺研究.pdfVIP

Mycosystema 菌 物 学 报 15 September 2010, 29(5): 760-766 jwxt@ ISSN1672-6472 CN11-5180Q ©2010 Institute of Microbiology, CAS, all rights reserved. 正交实验法优化桦褐孔菌多酚类物质的提取工艺研究 * 张梅梅 郑维发 赵艳霞 刘玉冰 魏志文 徐州师范大学江苏省药用植物生物技术重点实验室 徐州 221116 摘 要：以乙醇为提取溶剂，通过正交实验对影响桦褐孔菌多酚提取的乙醇浓度、提取时间、提取温度和料液比等因素进行 探讨。结果显示，桦褐孔菌多酚的乙醇提取优化工艺参数为：乙醇的浓度为 50%，料液比为 1:10，温度为 50℃，提取时间 为 45min ，得到的多酚提取率可达到 3.099% 。用乙醇-水混合体系提取桦褐孔菌多酚是一种安全、经济、有效的方法。 关键词：桦褐孔菌，多酚，正交试验，乙醇水溶液 Optimization on extraction of polyphenols from sclerotia of Phaeoporus obliquus by orthogonal test method * ZHANG Mei-Mei ZHENG Wei-Fa ZHAO Yan-Xia LIU Yu-Bing WEI Zhi-Wen Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China Abstract: Aqueous ethanol was used as solvent to optimize the conditions for maximum extraction of polyphenols from sclerotia of Phaeoporus obliquus by orthogonal test method in which ethanol concentration, extraction time, temperature and the volume ratio of material and solvent were taken into account as factors. The results showed that the maximum extraction of polyphenols (3.099% m/m) was obtained by 50% aqueous ethanol with a ratio of material and solvent at 1:10, extraction temperature at 50℃ and extraction time at 45min. Aqueous ethanol is a safe, economical and effective solvent for extracting polyphenols from Paeoporus obliquus . Key words: Phaeoporus obliquus, polyphenol, orthogonal experiment, aqueous ethanol 桦褐孔菌 Phaeoporus obliquus (Pers.:Fr.) J. 木的树皮下或砍伐后树木的枯干上（Saar 1991；黄 Schroet.，是一种生长在寒带的木腐菌，属于真菌门、 年来 2002 ）。十六世纪至今，桦褐孔菌一直作为民 担子菌亚门、层菌纲、非褐菌目、多孔菌科、褐卧 间药物，在预防和治疗癌症、心脏病、肝病、糖尿 孔菌属。主要分布于北纬 45 －