采用连续搅拌棒吸附萃取热脱附和GCMS分析筛查水中的农药….pdfVIP

采用连续搅拌棒吸附萃取–热脱附和 GC/MS 分析筛查水中的农药残留 Application Note Food Authors Abstract Nobuo Ochiai and Kikuo Sasamoto 本文介绍了连续搅拌棒吸附萃取（连续 SBSE ）结合热脱附（TD ）-GC/MS 方法筛查水中 GERSTEL K.K. 农药残留的性能。相比传统的 SBSE ，连续 SBSE 能够在整个极性和挥发性范围内对水中 2-13-18 Nakane 超痕量级的有机污染物实现更为均一的富集。连续 SBSE 的过程如下：首先使用一根搅拌 棒对 5 mL 不含改性剂的样品实施 SBSE ，然后在同一样品中添加 30% NaCl 后使用第二根 Meguro-ku, Tokyo, 152-0031 搅拌棒再次进行 SBSE 萃取后，将两根搅拌棒置于一根玻璃脱附衬管中，同时进行热脱 Japan 附。通过保留时间锁定的 GC/MS 方法（RTL-GC/MS）可证明农药的存在。本研究采用 连续 SBSE-TD-RTL-GC/MS （在扫描模式下运行）对河水样品中 ng/L 级的农药进行了成 功筛查。 Introduction Sequential SBSE-TD-RTL-GC/MS For the first SBSE, five milliliters of water sample were trans- The determination of pesticide residues in environmental ferred to 10 mL headspace vials. A stir bar (Gerstel Twister; samples, such as water, soil, and agricultural products, has 24 µL of PDMS) was added and the vial was sealed with a been a major subject for many years. This is because of their screw cap. SBSE of several samples was performed simulta- potential risk for human health, persistence and tendency to neously at room temperature (24 ºC) for 60 min while stirring bio-accumulate. For water samples, analytical methods usual- at 1500 rpm. After the first extraction, the stir bar was ly include extraction and enrichment steps for determining removed with forceps, dipped briefly in Milli-Q water, dried pesticide residues at very low levels (sub-µg/L). Therefo