凝胶净化-气相色谱-质谱法同时测定羊羔不同.docVIP

凝胶色谱净化-气相色谱-质谱法同时测定烧烤肉 制品中19种多环芳烃 马 玲1,张旭龙2,刘 银1,王宽强1,彭 秧1* （1. 新疆大学 化学化工学院，乌鲁木齐 830046；2. 新疆出入境检验检疫局，乌鲁木齐 830063） 摘 要：建立了一种气相色谱-质谱(GC-MS)法测定烧烤肉制品中19种多环芳烃(PAHs)的方法。通过优化前处理条件，选择正己烷-二氯甲烷作为提取剂，匀浆法提取，上清液经凝胶色谱净化后用GC-MS对目标化合物进行测定。各化合物线性相关系数r0.998；19种PAHs方法精密度RSD21.4%；回收率为58.0%～121.5%。采用本方法对市售的不同羊羔器官烧烤制品进行检测,样品中PAH浓度为0.3～74.7 μg?kg-1。本方法灵敏度高，简便快捷，能同时测定烤肉制品中19种多环芳烃。 关键词：多环芳烃；烧烤肉制品；凝胶色谱；气相色谱-质谱法 中图分类号：O657 Simultaneous Determination of Nineteen Polycyclic Aromatic Hydrocarbons in Grilled Meal Samples by GPC-GC-MS MA Ling1, ZHANG Xu-long2, LIU Yin1, WANG Kuan-qiang1, PENG Yang1* （1. College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046, China; 2. Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi 830063, China) Abstract: A method was established for simultaneous determination of nineteen polycyclic aromatic hydrocarbons (PAHs) in grilled meal samples using gas chromatography-mass spectrometry (GC-MS). Through the optimization of pre-treatment conditions, the samples were extracted with n-hexane - dichloromethane and then purified by gel permeation chromatography (GPC). All these compounds were determined by GC-MS. The results showed that the calibration curves had good liner relationship (r0.998). The recovery ranges were from 58.0% to 121.5% with the relative standard deviations (RSD) was less than 21.4% for PAHs. The method was then applied to the determination of PAHs in different parts of the lambs barbecue. 19 PAHs were found to be present at levels ranging from 0.3 to 74.7μg/kg. The method was accurate, simple and rapid to analyze 19 PAHs in grilled meal samples. Keywords: Polycyclic aromatic hydrocarbons; Grilled meal samples; Gel permeation chromatography; Gas chromatograph-mass spectrometer 多环芳烃(PAHs)是重要的环境和食品污染物，其中相当部分化合物具有致癌性[1]，如苯并(a)蒽，苯并(a)芘等。食品中的PAHs来源于加工过程中有机物热解和不完全燃烧， 由于烤肉制品具有一定脂肪,因此烧烤类食品常含有一定量PAHs。长期食用烧烤肉制品对健康产生潜在威胁。 目前，烤肉中PAHs的检测方法主要有气相色谱法和高效液相色谱法及其与质谱联用技术[2-8]。上述研究方法的对象主要是肉类，对于动物源性产品中的其它器官（如心、肠、板筋等）烧烤制品中PAHs含量的测