苹果果实发酵液对草莓防御酶系活性及抗病性影响.pdfVIP

中国农学通报 2010,26(16):250-255 Chinese Agricultural Science Bulletin 苹果果实发酵液对草莓防御酶系活性及抗病性的影响 1 1 1 2 2 1 2 王荣娟 ，戚亚平 ，宋备舟 ，黄 彬 ，王 雷 ，姚允聪 ，姬谦龙 1 2 （北京农学院植物科学技术学院，北京102206；北京农学院生物技术系，北京102206） 摘 要：探讨在有机栽培方式下，苹果果实发酵液诱导草莓抗灰霉病（Botrytis cinerea ）的作用。用4 个处 理喷施草莓，5 天后接种灰霉病病菌。接菌6 天后统计不同处理的病斑数，同时测定过氧化物酶（POD ）、 多酚氧化酶(PPO)及β-1,3- 葡聚糖酶在接种后12 天内的动态变化。Tr2 处理后，草莓的抗病指数达到 34.6% ，显著高于对照，其他处理差异不显著。喷施Tr 1、Tr2、Tr3 可使POD 、PPO 酶活性高于对照，β-1,3- 葡聚糖酶活性高峰出现较对照早。苹果果实发酵液100倍稀释液和0.02 mol/mL 水杨酸混合喷施可诱 导草莓对灰霉病产生抗性。这对草莓有机生产中草莓灰霉病的防治提供了新途径。 关键词：苹果果实发酵液；草莓；灰霉病；水杨酸；抗病性 中图分类号：S432.4 文献标志码：A 论文编号：2010-0873 Influences of Apple Fruit Broth on Activities of Defense Enzymes and Disease Resistance on Strawberry 1 1 1 2 2 1 2 Wang Rongjuan , Qi Yaping , Song Beizhou , Huang Bin , Wang Lei , Yao Yuncong , Ji Qianlong 1 2 ( College of Plant Science and Technology in BUA, Beijing 102206; Department of Biotechnology in BUA, Beijing 102206) Abstract: This paper explored the effect of the apple fruit broth on inducing resistance of strawberries to Botrytis cinerea under the organic cultivation. We sprayed strawberries with four different treatments. Then 5 days later, the strawberries were inoculated with Botrytis cinerea. 6 days after inoculating, the numbers of necrotic lesions were counted and activities of peroxidase, polyphenol oxidase and β-1, 3-glucanase were assaied. The results showed th