人UCA1 基因转录调控的生物信息学分析与鉴定.pdfVIP

·1596 · doi 10.3969/j.issn. 1673-4254.2013.11.07 J South Med Univ, 2013, 33(11): 1596-1599 技术方法 人人UCAUCA11基因转录调控的生物信息学分析与鉴定基因转录调控的生物信息学分析与鉴定 1 1 2 薛 梅 ，李 旭 ，陈 葳 1 2 西安交通大学医学院第一附属医院 转化医学中心，检验科，陕西 西安 710061 摘要：目的 生物信息学分析与鉴定人类膀胱癌特异性表达的UCA 1基因转录调控作用原理。方法 运用CpG 岛预测软件 EMBOSS CpGplot、CpG Island Searcher、Methprimer、CpG finder 对UCA 1基因启动子CpG 岛进行分析。利用转录因子预测软 件MatrixCatch 和TFSEARCH 对UCA 1基因核心启动子区可能结合的转录因子进行分析。染色质免疫沉淀技术对所预测到的 转录因子进行分子生物学实验验证。结果 CpG 岛预测发现UCA 1基因全长启动子区中不存在CpG 岛，因此UCA 1基因属于组 织特异性基因与前期研究报道结果一致。生物信息学预测及筛选发现UCA 1基因核心启动子存在4 个与肿瘤关系密切的转录 因子。根据预测结果选取2 个转录因子利用染色质免疫沉淀实验鉴定，确定1个转录因子可与UCA 1基因核心启动子区结合。 结论 UCA 1基因启动子区无CpG 岛存在，该基因核心启动子区可能与多种转录因子结合，并确定转录因子c-Myb 可以与 UCA 1基因核心启动子区结合。 关键词：生物信息学；UCA 1基因；转录调控；转录因子 Bioinformatics analysis and identification of transcriptional regulation of human UCA1 gene 1 1 2 XUE Mei , LI Xu , CHEN Wei 1Center for Translational Medicine, 2Department of Laboratory Medicine, First Affiliated Hospital of Xian Jiaotong University College of Medicine, Xian 710061, China Abstract: Objective To analyze the transcription factor binding sites and methylation in the promoter region of UCA1 gene. Methods UCA1 gene promoter was analyzed using CpG software to predict the CpG island. MatrixCatch and TFSEARCH were used to predict the potential transcription factor binding sites in UCA1 gene core promoter. ChIP assay was used to identify the transcription factors binding to UCA1 gene core promoter. Results UCA1 gene promoter contained no CpG island and was therefore a typical tissue-specific gene. There were 4 transcription factors associated with human cancers in UCA1 gene core promoter, but only one of them interacted with UCA1 gene core promoter. Conclusion There is no CpG island in