旋果苣组织培养的研究Ξ.pdfVIP

吉林农业大学学报 　2010 ,32 ( 1) :51～53 http :/ / x uebao. j lau . edu . cn J ournal of J ilin A gricultural University Email :j lndxb @vip . sina . com 旋果苣组织培养的研究 王 　薇, 钱 　浩, 陈志刚 吉林农业大学园艺学院 , 长春 130118 ( ) 的叶、花葶和花蕾为外植体 , 以MS 为基本培养基 ,研究了组织培养 摘 　要 : 以旋果苣 Streptocarp us wendlanddii 快繁培养基中细胞分裂素和生长素最佳添加量和不同外植体的最佳灭菌时间。结果表明:在以 1 g/ L HgCl2 溶液进行灭菌时 ,叶片的最佳灭菌时间是 4～5 min ,花葶 、花蕾的最佳灭菌时间是 4 min ;在用“84 ”消毒液进行 灭菌时 ,花葶 、花蕾的最佳灭菌时间是 5 min 。愈伤组织诱导和根分化的最佳培养基均为 MS + BA 10 mg/ L + NAA 10 mg/ L ;芽分化的最佳培养基为 MS + BA 40 mg/ L + NAA 02 mg/ L ;继代培养最佳培养基为 MS + BA 10 mg/ L + KT 05 mg/ L 。 关键词 : 旋果苣 ; 叶; 花葶 ; 花蕾 ; 组织培养 ( ) 中图分类号 : S68 　　　文献标识码 : A 　　　文章编号 : 2010 Studies on Tissue Culture of Streptocarpus wendlanddii WAN G Wei , QIAN Hao , CHEN Zhigang College of Horticulture , J ilin A gricultural University , Changchun 130118 , China Ab stract : Leaf , scape and bud of Strep tocarp us wendlanddii were used as explants in this study . Based on MS medium , the appropriate approach and sterilization time of tissue culture and rapid propagation of Strep tocarp us wendlanddii were obtained via regulating the concentrations of cytokinin and auxin . The re sults showed that when the explants were sterilized in 1 g/ L mercuric chloride , the optimum sterilization time for leaf was 4 —5 minutes , while bud was 4 minutes. When the explants were sterilized with 84 dis infectant liquid , the suitable time for scape and bud was 5 minutes. The optimal medium for callus in duction was MS + BA 10 mg/ L + NAA 10 mg/ L ;the optimal medium for bud differentiation was MS +