烟草细胞质雄性不育线粒体基因sdh3 和sdh4 的生物信息学分析.pdfVIP

42 Chinese Tobacco Science 2013-06 ，34 （3 ） sdh3 sdh4 1,2 1 1 1,2 1* 1. 266101 2. 100081 sdh3sdh4 II sdh3sdh4 3 DNA GenBank DNA sdh3sdh4 sdh4 28 sdh3 10 3 CMS sdh3 sdh4 S572.03 1007-5119201303-0042-06 DOI 10.3969/j.issn.1007-5119.2013.03.09 Bioinformatics Analysis of Mitochondrial Genes sdh3 and sdh4 of Cytoplasmic Male Sterility in Tobacco 1,2 1 1 1,2 1* ZHANG Changjing , LI Fengxia , JIA Xinghua , LI Zunqiang , FENG Quanfu (1.Tobacco Research Institute of CAAS, The Key Laboratory for Tobacco Gene Resources of State Tobacco Monopoly Administration, Qingdao 266101, China; 2.Graduate School of CAAS, Beijing 100081, China) Abstract: The abnormal mitochondrial gene may lead to tobacco cytoplasmic male sterility. sdh3 and sdh4 are important genes coding subunits of mitochondrial complex II. To study the relationship between mitochondrial genes sdh3, sdh4 and cytoplasmic male sterility, the mitochondrial DNA from 3 male sterile lines and their maintainer lines of Nicotiana tabacum cultivars were extracted, specific primers were designed according to the complete nucleotide sequence of the tobacco mitochondrial genome in GenBank to amplify purpose genes from tobacco mitochondrial DNA. PCR products were cloned and sequenced. The sdh3 and sdh4 nucleotides and encoded protein structures were analyzed with bioinformatics to find differences between male sterile lines and maintainer lines. The results showed that the 28th base of sdh4 of male sterile lines gene changed. The sdh3 of male sterile l