咪达唑仑对新生大鼠离体脊髓运动神经元作用.docVIP

咪达唑仑对新生大鼠离体脊髓运动神经元的作用* 宦才娟，汪萌芽* （皖南医学院细胞电生理研究室，安徽 芜湖241002） Effects of midazolam on motoneurons in neonatal rat spinal cord in vitro HUAN Cai-juan, WANG Meng-ya* Cell Electrophysiology Laboratory, Wannan Medical College, Wuhu, Anhui 241002, China [Abstract] Objective: To observe the effects of midazolam on motoneurons (MNs) in neonatal rat spinal cord slices. Methods: The conventional intracellular recording techniques were used to observe the effects of midazolam at distinct concentration on MNs of spinal cord slices isolated from neonatal rats (8~14d old). Results: In the 5 MNs steadily recorded, not only a depolarization response of (18.2±10.8) mV with increase of membrane resistance was induced by perfusion of midazolam at 0.1-1.0 mmol/L for 15 min of each concentration (, P0.05 or P0.01), but also amplitude of action potentials was decreased (P0.05), and even action potentials were nullified in 2 MNs. In 4 MNs with dorsal rootlet stimulation-evoked excitatory postsynaptic potentials (DR-EPSPs), bath of midazolam (0.1-1.0 mmol/L) reversibly depressed DR-EPSPs in a concentration-dependent manner. Moreover, in 3 MNs with DR-EPSP followed by inhibitory postsynaptic potentials (DR-IPSPs), DR-EPSPs were inhibited and DR-IPSPs were enhanced by midazolam at low concentration, whereas both of them were nullified at high concentration. Conclusion: The findings suggest that midazolam may reversibly and concentration-dependently inhibit the MN excitability and its synaptic transmission from sensory inputs. [Key words]: spinal cord; motoneuron; synaptic transmission; midazolam; electrophysiology [摘 要] 目的: 观察咪达唑仑对新生大鼠离体脊髓运动神经元（MN）的作用。方法: 采用常规细胞内记录技术，观察不同浓度的咪达唑仑对新生大鼠（8~14d）离体脊髓MN的作用。结果: 在5个记录稳定的MN，累积灌流0.1 ~ 1.0 mmol/L咪达唑仑各15 min，产生去极化反应 (P005或P0.01)，并伴有膜电阻的增大(P005)，而且抑制动作电位的幅度和发放频率(P0.05)，且1.0 mmol/L的咪达唑仑在其中2个MN完全取消动作电位的产生。在4个记录到背根刺激诱发出兴奋性突触后电位（DR-EPSP）的MN，0.1 ~ 1.0 mmol/L咪达唑仑累积灌流各15 min，亦呈现浓度依赖性抑制作用，其中0.1 mmol/L的咪达唑仑对DR-EPSP抑制性作用具有统计学意义(n=3, P0.05)。而在记录到DR-EPSP合并有抑制性突触后电位