海南龙血树RAPD-PCR反应体系优化.pdfVIP

Guihaia 31 ( 1) : 31 - 35 2011 1 DOI: 10. 3969/j. issn. 1000-3142. 2011.01. 007 RAPD-PCR 郑道君, 谢良商, 张 文, 张治礼* ( , 571100 ) 2+ : ,RAPD-PCR DNA Mg dN P , aq , RAPD-PCR , RAPD-PCR , 25 LL , 10 @ buffer 2. 5 LL, 2. 0 mmol/L MgCl , 300 2 Lmol/ L dN Ps, aq 1. 5U ,0. 8 Lmol/L DNA 20 ng, RAPD-PCR 18 , RAPD : ; RAPD; ; : Q943 : A : 1000-3142( 2011)01-0031-05 ¹ Optimization of RAPD-PCR reaction system for Dracaena cambodiana * ZHENG Dao-Jun, XIE Liang-Shang, ZHANG Wen, ZHANG Zh-i Li ( K ey Laboratory of Crop Genetics and Breeding, H ainanA cademy of A gr icultural Sciences , Haikou 571100, China ) Abstract: Both single factor test and orthogonal design were applied to study the effects of main factors on the RAPD- PCR system for Dracaena cambod iana,in which the main factors included the content of template DNA, the concen- 2+ tration of Mg dN Ps andprimers and the content of aq DNA polymerase, and an optimal 25LL RAPD-PCRreac- tion system for D. cambod iana was established, including 2.5 LL 10@ PCR buffer,2. 0 mmol/ L MgCl , 300 Lmol/L 2 dN Ps, 1.5 U aq polymerase, 0. 8 Lmol/L primers, and 20 ng DNA template. 18 primers were selected for RAPD- PCR analysis for D. cambodiana using the optimized amplification system above,which can be employed for the analy- sis of genetic variation and structure of D. cambodiana population. Key wor