连翘酯苷对IFN-α 和Mx1 表达的影响.pdfVIP

中国农业科学 2010,43(15):3237-3243 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.15.023 连翘酯苷对IFN-α和Mx1表达的影响 马元元，张中文，李华伟，孙健华，许承扬，吴国娟 （北京农学院动物科学技术系，北京 102206 ） 摘要： 【目的】探讨连翘酯苷作用于感染 PCV2 的小鼠模型后，对 IFN-α和 Mx1 表达的影响，以评价连翘酯 苷的抗病毒作用。【方法】复制PCV2感染小鼠模型。以（2、4、10 mg·mL-1)连翘酯苷分为高、中、低 3个剂量组， 并设空白对照组和病毒对照组。给药12、24、36 h后，分别提取小鼠肺组织总RNA和总蛋白，应用实时荧光定量 PCR 检测 IFN-α、Mx1 mRNA 含量，采用 Western blotting 检测 Mx1 蛋白的表达水平。【结果】空白对照组小鼠 肺组织中 Mx1 和 IFN-α无表达，病毒对照组少量表达。随着连翘酯苷浓度的升高，IFN-α和 Mx1 mRNA 水平逐步 升高，两者的表达量呈现平行相关；高剂量组中IFN-α和Mx1表达量最高(P ＜0.001)；随着药物作用时间的延长 表达量降低，12 h 时达到最高（P ＜0.001），其后逐渐下降。【结论】昆明小鼠适于 PCV2 感染模型的建立。中 药连翘酯苷能显著上调IFN-α和Mx1的表达，并在给予高剂量药物后12 h发挥显著的抗病毒作用。Mx1蛋白与病 毒的感染密切相关，可用于病毒感染的早期诊断，及具有较好的应用前景。 关键词：连翘酯苷；IFN-α；Mx1；PCV2 Effects of Forsythoside A on the Expression of IFN-α and Mx1 MA Yuan-yuan, ZHANG Zhong-wen, LI Hua-wei, SUN Jian-hua, XU Cheng-yang, WU Guo-juan (Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206) Abstract: 【Objective 】 The purpose of the experiment is to research the effects of forsythoside A on the expression of IFN-α and Mx1 in the mouse model of PCV2 infection, and further to evaluate the antiviral activity of forsythoside A. 【Method 】 The mouse models were established by infection with PCV2 and then divided into low, medium and high-dose groups, which were treated with 2, 4, 10 mg·mL-1 forsythoside A respectively, the blank control group and virus control group. Total RNA and total protein were extracted from the lung tissue of mouse in each group after 12, 24, 36 h administration. The mRNA content of IFN-α and Mx1 were detected by Real-time fluorescence quant