参芪苈心方对心肌细胞线粒体的保护作用.pdfVIP

中国科技论文在线 参芪苈心方对心肌细胞线粒体的保护作用# 胡鸣旭，刘莉** （黑龙江中医药大学附属第一医院心内科，哈尔滨 150040） 5 摘要： 目的：观察参芪苈心方对去甲肾上腺素诱导H9C2 大鼠心肌细胞线粒体的保护作用， 探讨参芪苈心方保护心肌细胞的作用机制。方法：H9C2 大鼠心肌细胞分为正常对照组、模 型组、盐酸曲美他嗪组，参芪苈心方组。后 3 组依次加入正常大鼠血清 10μL、盐酸曲美他 嗪含药血清 10μL、参芪苈心方含药血清 10μL ，各组均加入去甲肾上腺素（最终浓度均为 2μmo1/L ）,在加药后 24 小时，进行指标检测，心肌细胞线粒体膜电位变化及心肌细胞凋亡 10 率。结果：参芪苈心方能维持受损心肌细胞线粒体膜电位的稳定性,抑制心肌细胞凋亡，优 于西药盐酸曲美他嗪组。结论：参芪苈心方能通过维持心肌细胞线粒体膜电位的稳定性来抑 制受损心肌细胞凋亡，保护心肌细胞，这可能是参芪苈心方治疗充血性心力衰竭机制之一。 关键词：H9C2 心肌细胞；参芪苈心方；线粒体膜电位; 凋亡 中图分类号：R242 15 the protective effect of Shenqilixin Recipe on mitochondria of cardiomyocytes Hu Mingxu, Liu Li (No.1 hospital of heilongjiang university of TCM, ward of cardiology, Harbin 150040) 20 Abstract: Objective: To observe the effect of Shenqilixin Recipe on the energy metabolism of H9C2 cells induced by norepinephrine , and explore the treatment mechanism of Shenqilixin Recipe on heart failure. Methods: H9C2 rat cardiomyocytes were divided into normal control group, model group, trimetazidine group and Shenqilixin group. The last three groups were added with normal rat serum 10μL, serum with 10μL trimetazidine, serum with 10μL Shenqilixin Recipe 25 and each group added norepinephrine (final concentration was 2μmo1 / L), indicators tested at the 24 hour-cardiomyocytes mitochondrial membrane potential and apoptosis rate. Results: Shenqilixin Recipe could maintain the stability of the mitochondr