TRPM7在dHL-60细胞极性化中的作用.pdfVIP

中国科技论文在线 # TRPM7 在dHL-60 细胞极性化中的作用 吴娴波，邹文英，蔡春青，陈新宏，邹飞，孟晓静** （南方医科大学公共卫生与热带医学学院，广州510515 ） 5 摘要：目的 探讨TRPM7 对dHL-60 细胞极性化的影响及可能的分子机制。方法 通过siRNA 技术下调dHL-60 细胞TRPM7 蛋白表达，用zigmond 小室观察细胞极性化的变化，并采用 Western blotting 方法检测TRPM7 和磷酸化AKT 的表达水平。结果 转染TRPM7 靶向siRNA 的dHL-60 细胞中，TRPM7 蛋白表达水平明显降低 （P0.05 ）；干扰TRPM7 后dHL-60 细 胞的极性化率显著增加；磷酸化 AKT 表达明显上调，而总AKT 的表达则无明显变化。结 10 论 TRPM7 通过AKT 信号通路调控细胞极性相关分子，进而影响dHL-60 细胞的极性化能 力。 关键词：TRPM7；dHL-60 细胞；极性化；AKT 中图分类号：Q256 15 Effect of TRPM7 gene silencing on differentiated HL-60 cell polarization WU Xianbo, ZOU Wenying, CAI Chunqing, CHEN Xinhong, ZOU Fei, MENG Xiaojing (School of Public Health and Tropical Medicine, Guangzhou 510515) 20 Abstract: Objective To determine the effect of TRPM7 gene silencing on differentiated HL-60 (dHL-60) cell polarization and its possible molecular mechanism. Methods Cell polarization was observed in dHL-60 cell transfected with a small interfering RNA (siRNA) targeting TRPM7. Western blotting was used to examine the expressions of TRPM7 and phosphorylated AKT in the transfected cells. Results Transfection of dHL-60 cells with TRPM7-specific siRNA significantly 25 decreased the proteins levels of TRPM7, increased cell polarization and the expression of phosphorylated AKT(P0.05). However, the expression of AKT was not affec