玻璃电泳芯片测试问题研究.pdfVIP

中国科技论文在线 玻璃电泳芯片测试问题研究 艾洪新，何越，荣吉赞，杨楠** （凯晶生物科技 （苏州）有限公司，苏州） 5 摘要：本文用单色荧光素罗丹明110 （RH110 ）以电渗原理测试玻璃电泳芯片，所用的方法 是激光诱导荧光检测方法。在玻璃电泳芯片上，出现的一些问题进行研究，结果证明芯片 清洗过程中，用缓冲液清洗微通道会使基线平稳；电泳电压的简单计算公式有效，能够优 化峰形；储液池液面高度保持一致对于峰形的有益影响。通过一段时间的测试，初步掌握 了玻璃电泳芯片的一些规律。 10 关键词：玻璃电泳芯片；激光诱导荧光检测；罗丹明110；芯片清洗；电压计算；储液池液 面 中图分类号：O657 Problems appeared in the elecrophoresis process of glass 15 electrophoresis chip AI Hongxin, HE Yue, RONG Jizan, YANG Nan (ChemoGene Biotechnologies Suzhou Ltd,Suzhou) Abstract: In this paper the glass electrophoresis chip was test using the RH110 according to the electroosmosis principle . The method was the laser-induced fluorescence detection. Some problems 20 appeared were researched in the glass electrophoresis chip and the results demonstrated that chip cleaned with the electrophoresis buffer could make the base line stable;a simple formula to calculate voltage of electrophoresis was effective, which could optimize peak shape; Fluid level of liquid pools stayed the same, which would be good for peak shape. Some rules of glass electrophoresis chip was grasped after a series of experiment . 25 Key words: Glass electrophoresis chip; laser-induced fluorescence detection; RH110; chip cleaning; calculating voltage;Fluid level of liquid pools. 0 引言 毛细管电泳芯片是近几年出现的微量分析装置，主要应用于蛋白质组学研究、基因临床 30 诊断、药物筛选、DNA 测序及法医鉴定等领域。芯片中毛细管的内径一般在几十微米到100 微