基于淬灭荧光素的一种TCSPC仪器响应函数测量方法.pdfVIP

中国科技论文在线 基于淬灭荧光素的一种TCSPC 仪器响应函 数测量方法# 梁荣荣，陶占东，刘梦薇，潘海峰，张三军，徐建华** 5 （华东师范大学精密光谱科学与技术国家重点实验室，上海 200062 ） 摘要：通过测量仪器响应函数（IRF ）及重新卷积可以提高荧光寿命测量中的时间分辨率。 根据碘离子对荧光物质的动态淬灭作用原理，将碘化钠饱和水溶液按照一定的比例加入到 碱性荧光素水溶液中，并分别使用具有皮秒时间分辨的TCSPC 设备和飞秒分辨的荧光频率 10 上转换设备对淬灭后的荧光素荧光寿命进行测量。实验结果表明，随着碘化钠浓度的增 加，荧光素寿命从4ns 减小至24ps 左右。将淬灭后的荧光素作为仪器响应函数参考染料与 二氧化硅悬浮液进行对比，发现二者的结果几乎完全相同。此研究不仅解决了时间分辨激 发光谱测量中采用散射样品测量需在不同探测波长反复测量 IRF 的问题，更重要的是避免 了实验中由系统颜色效应带来的误差。 15 关键词：时间分辨荧光光谱；荧光素；碰撞淬灭；仪器响应函数 中图分类号：O433 TCSPC Instrument Response Function measurement by Quenched Fluorescein 20 LIANG Rongrong, TAO Zhandong, LIU Mengwei, PAN Haifeng, ZHANG Sanjun, XU Jianhua (State key laboratory of precision spectroscopy, East China Normal University, ShangHai 200062) Abstract : Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide 25 ions have been successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra 30 measurements, the IRF should be measured at various detection wavelengths providing scatting materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system.