20应用全二维气相色谱飞行时间质谱分析烟草中性化学成分.pdfVIP

20. 应用全二维气相色谱飞行时间质谱分析烟草中性化学 成分 1 2 2 1 1 1 鹿洪亮 ,赵明月 ,刘惠民 ,吴正举 ,何阳明 ,梁俐俐 (1．福建中烟工业公司技术中心，福建省厦门市海沧区新阳工业区新阳路1号 361022； 2．中国烟草总公司郑州烟草研究院，郑州市高新技术产业开发区枫杨街2号 450001） 摘要：首次应用全二维气相色谱飞行时间质谱(GC×GC-TOFMS)研究了烟叶的中性化学成分，考察了色谱柱系统、操作条 件（调制周期、柱头压力和初始温度等）对两维分离的影响，建立了烟草挥发性、半挥发性中性化学成分分析的 GC×GC-TOFMS方法。以云南楚雄产云烟85C3F烟叶为例，进行了GC×GC-TOFMS与GC-MS在定性方面比较，结果显示： 采 用 GC-MS 方法共鉴定出 73 种成分，而 GC×GC-TOFMS 方法共鉴定出 569 种成分，定性结果更加可靠。同时比较了它们的 峰容量、分离能力和灵敏度，结果显示：GC×GC-TOFMS的峰容量是GC-MS的22倍，灵敏度提高了10-82倍；在1DGC-MS 上峰形很好的一个峰，被GC×GC-TOFMS分离出6种成分。还以C1、C2、C3、C4取代萘为例对GC×GC-TOFMS在族分离方 面的能力进行了研究。 关键词：全二维气相色谱飞行时间质谱、烟草、中性化学成分、同时蒸馏萃取 Analysis of Semi-Volatile Neutral Constituents in Tobacco by GC×GC-TOFMS Lu Hong-Liang(1), Zhao Ming-Yue(2), Liu Hui-Min(2), Wu Zhengju(1), He Yang-ming(1), Liang Li-Li(1) 1. RD Center of Fujian Tobacco Industrial Corporation, Fujian,361022, China 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Abstract: GC×GC-TOFMS was used to analyze tobacco for the first time. The operational conditions of GC×GC-TOFMS were studied, column sets, modulation frequency, column pressure and initial temperature were optimized. Yun85C3F, a flue-cured tobacco, was analyzed in detail. The results showed that GC×GC-TOFMS was more powerful than GC-MS in qualification and quantification studies. 73 constituents were identified in the neutral fraction by GC-MS, whereas 569 constituents were identified by GC×GC-TOFMS. GC×GC-TOFMS was more accurate than GC-MS in identifying unknown compounds; a peak i