高压液相色谱法测定柑橘类水果中的+柑橘红+2+号染料.pdfVIP

4 5 Vol. 4 No. 5 2013 10 Journal of Food Safety and Quality Oct. , 2013 2 * 胡 莉 , 仲伶俐, 郭灵安, 杨晓凤, 毛建霏, 李 曦, 付成平, 赵泓洋 (, 610066) 摘 要: 目的 (HPLC) 2 方法 , NH2 , Waters Sunfire C18(250 mm×4.6 mm, 5.0 µm), - , , 1.0 mL/min. 结果 : 0.5~5.0 µg/mL (r=0.99973), 0.003 mg/kg, 0.01 mg/kg6 , 2 mg/kg 3 , 79.48 %~93.20%, 6. 5% ~ 14.5 %36 , , 3 结论 , , 2 关键词: 2 ; ; Determination of citrus red 2 in citrus by high perssure liquid chromatography * HU Li , ZHONG Ling-Li, GUO Ling-An, YANG Xiao-Feng, MAO Jian-Fei, LI Xi, FU Cheng-Ping, ZHAO Hong-Yang (Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China) ABSTRACT: Objective To establish a rapid method for the determination of citrus red 2 (CR2) in citrus by high pressure liquid chromatography (HPLC). Methods The samples were extracted with acetonitrile, cleaned-up by NH2 solid phase extraction cartridge. Then, the samples were separated on a Waters Sunfire C18 (250 mm×4.6 mm, 5.0 µm) in gradient elution with the mobile phases of water and acetonitrile. The separated compounds were detected with UV/VIS detector. The linearity of the method (r = 0.99973) was good over the concentration range from 0.5 to 5.0 µg/mL for CR2. The limit of detection (LOD) was 0.003 mg/kg, and the limit of quantification (LOQ) was 0.01 m