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毒鼠强对大鼠脑细胞色素C表达的影响
何跃军
(武警8730部队医院法医鉴定科,广东广州510800)
【摘? 要】目的:探讨毒鼠强慢性中毒的大鼠脑部细胞色素C的表达情况。方法:建立SD大鼠毒鼠强慢性中毒模型,检测中毒后不同时间大鼠脑部细胞色素C的表达。结果:对照组大鼠脑部细胞色素C仅有微量表达,实验组大鼠中毒后30分钟细胞色素C的表达开始增强,1-3D达高峰,4-5D后开始下降,10D后基本恢复到正常水平。结论:大鼠细胞色素C随时间的变化可为推断毒鼠强慢性中毒的不同时期提供法医学证据。
【关键词】毒鼠强;细胞色素C;慢性中毒;免疫组化
毒鼠强(TETRAMINE,TETS)的化学名是四甲基砜四胺,系有机氮化物,为白色轻质粉末,属于剧毒物品,目前尚无特效解毒药。
Expression of Cyt C in Brain of Rats Poisoned by Tetramine
He Yuejun
Department of Forensic Identification, Hospital of 8730 Armed Forces, Guangzhou, Guangdong 510800.
【Abstract】Objective:To explore the evidence for the judgement on legal cases of intoxation by tetramine. Method:Sixty rats given tetramine by lavage were sacrificed at different time after intoxation.The expression of Cyt C was detected by immunohistochemistry,and the reasults were assessed by an image analysis system. Results:There was a slight expression of Cyt C in control group.In intoxation group the expression of Cyt C increased after the rats were given tetramine for 30 minutes;it reached the peak at 24 hours after intoxation and kept up the level for 3 days,then it began to decrease and returned to the control level in 1 0 days.Conclusion:The regular change of Cyt C expression might be a useful sign for assess-
ment in legal cases of intoxation by tetramine.
【Key words】Tetramine;Cytochrome C;Chronic poisoning;Immunohistochemisty
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