大剂量维生素C对DNA损伤及槲皮素-7-硫酸酯钠的保护作用.pdfVIP

248 Journal of Medical Science in Central South China,May2012,Vol.40,No.3 文章编号:2095-1116(2012)03-0248-05 ·基础医学· 大剂量维生素C对 DNA损伤及槲皮素鄄7鄄 硫酸酯钠的保护作用 1 1 1 2 2 1 赵小燕 ,沈渠深 ,左长清 ,林摇 晓 ,潘文嘉 ,戴摇 忠 (1.广东医学院药理教研室,广东 东莞523808;2.广东医学院化学与药学实验教学中心) 摘摇 要:摇 目的摇 探讨大剂量维生素C(VC)对小牛胸腺DNA损伤及槲皮素鄄7鄄硫酸酯钠的保护作用。 摇 方法 小牛胸腺 DNA 用VC(0.1、0.25 及0.50 mmol/ L)预处理和用不同剂量槲皮素、槲皮素鄄7鄄硫酸酯钠(0.1、0.25、 0.50 mmol/ L)与VC(0.50 mmol/ L)以不同方式处理(预处理、同时处理),采用琼脂糖凝胶电泳技术检测分析DNA 电泳条带及用分光光度法检测分析DNA 浓度变化情况。 摇 结果摇 低剂量VC组DNA条带及DNA 浓度没有明显 变化;中高剂量VC组DNA条带明显拖尾,DNA 浓度显著升高;各浓度槲皮素鄄7鄄硫酸酯钠不同处理组DNA条带拖 尾现象较VC损伤组均减弱,小牛胸腺DNA条带变亮,DNA 浓度也有明显下降。 摇 结论摇 大剂量VC 能直接损伤 小牛胸腺DNA,槲皮素鄄7鄄硫酸酯钠对大剂量VC诱导的DNA损伤有保护作用,且呈明显浓度依赖关系。 关键词:摇 槲皮素鄄7鄄硫酸酯钠;摇 槲皮素;摇 维生素C;摇 DNA 中图分类号:R977.23摇 摇 摇 文献标识码:A Protective Effect of Sodium Quercetin鄄7鄄mono鄄sulfonate on High鄄dose Vitamin C Induced DNA Damage ZHAO Xiaoyan,SHEN Qushen,ZUO Changqing,et al (Department of Pharmacology,Guangdong Medical College,Dongguan,Guangdong 523808,China) Abstract:摇 Objective摇 To investigate the damage effect of high鄄dose Vitamin C(VC)on calf thymus DNA and the protective effect of sodium quercetin鄄7鄄sulfonate. 摇 Methods摇 Calf thymus DNA incubated with VC (0. 1,0. 25, 0.50 mmol/ L;quercetin and sodium quercetin鄄7鄄sulfonate were pretreated or co鄄treated with VC (0.50 mmol/ L) in calf thymus DNA.The changes of DNA were analyzed by agrose gel electrophoresis and spectrophotometry.摇 Results摇 The DNA ladder and DNA concentration had no obvious changing in0.10 mmol/ L VCgroup,but DNA ladder disappeared and the concentration of DNAobviouslyincreasedin0.25 and0.50 mmol/ LVCgroups.Pretreatmento