稀土元素铈对镉致泥鳅红细胞核异常的影响.pdfVIP

安徽农业科学,Journal of Anhui Agri .Sci .2008 ,36( 19) :8221- 8222 责任编辑 罗芸 责任校对 李洪 稀土元素铈对镉致泥鳅红细胞核异常的影响 田志环 ( 德州学院生物系, 山东德州253023) 摘要 [ 目的] 为治理镉污染提供理论依据 。[ 方法] 以泥鳅为材料, 以空白为对照, 设置了不 同浓度的镉铈处理, 观察处理24 h 后泥鳅 的 血 红细胞形态异常的数量 。[ 结果] 与对照组相 比, 染毒泥鳅的血 红细胞有较 大变异。在 Cd2 + 及 Cd2 + + Ce3 + 胁迫下 泥鳅 的血 红细胞 出 现粘连、凹陷等现象, 细胞核有微核 、破碎 、空泡、外凸等现象。不 同浓度的镉均能造成泥鳅红血细胞核异常, 随Cd2 + 浓度增大, 核异常率 显著增加; 当加入相 同浓度的Ce3 + 后, 低浓度组核异常率显著下降; 中浓度组核异常率亦有所下降; 而高浓度组核异常率明显上升 。[ 结 论] 低浓度的Ce3 + 能缓解 Cd2 + 造成的细胞核损伤, 而高浓度的Ce3 + 对 Cd2 + 造成的细胞核损伤则具有协 同作 用。 关键词 铈; 镉; 血 红细胞 中图分类号 S966 .4 文献标识码 A 文章编号 0517 - 6611(2008) 19 - 08221 - 02 The Influence of Lanthanide Ce on the Nuclear Anomalies of Blood Erythrocytes Caused by Cd in Loach TI AN Zhi- huan ( Biology Deparment , Dezhou College , Dezhou , Shandong 253023) Abstract [ Obj ective] The study was to provide a theoretical base for the control of Cd pollution . [ Method] With loach as tested material and no treatment as CK, treatments with Ce and Cd at different concn . were designed to observe the abnor mal number of blood erythrocytes in loach after treated for 24 h . [ Re- 2 + 2 + 3 + sult] Co mpared with CK, the blood erythrocytes in loach exposuredto toxicant had a big variation . Under Cd and Cd + Ce stress , the blood erythrocytes had phenomena such as adhesion , depression , while the cell nuclear had phenomena such as micronucleus , fragmentation , cavitation and outer convex . Treat- ments with Cd at different concn all caused the anomaly of blood erythrocytes nuclear . With the increase of Cd concn . , the abnormal rate of nuclear increased obviously . After Ce3 + at the same concn . was added , the nuclear abnor mal rates in treat