不同来源牛体外胚胎与牛子宫内膜细胞共培养体系建立.docVIP

不同来源牛体外胚胎与牛子宫内膜细胞共培养体系的建立 张 彧1,2，陈利平1，梁鸿斌2，李娅杰1，赵雅琦1，阎 芃1，杨 菲1，倪和民1，郭 勇1 （1 北京农学院动物科学技术学院，北京 102206；2北京奶牛中心，北京 100192） 摘 要：为了初步建立有效的牛胚胎与子宫内膜细胞的体外共培养体系。本实验分别将体外受精第7 天后的胚胎和孤雌激活，与培养7 天的胚胎与子宫内膜细胞共培养，在48、72 h后分别统计体外受精实验组和孤雌激活实验组的孵化率。实验结果表明：在本实验室培养条件下，在与子宫内膜细胞共培养48 h后，体外受精胚胎和孤雌发育胚胎在孵化率上存在显著差异(40±5)%、(25±5)%，(P＜0.05)；与子宫内膜共培养72 h 后，孵化率无显著差异(70±8.66)%，(65±5)%，(P＞0.05)。本实验证明了在体外环境下，牛体外胚胎与子宫内膜细胞共培养后能够正常孵化，可以初步建立起牛体外胚胎与子宫内膜细胞的体外共培养体系，并为进一步研究该体系对牛体外胚胎培养质量的影响奠定一些基础。 关键词：牛体外胚胎、子宫内膜细胞、体外共培养 中图分类号：S814.8 文献标志码：A 论文编号：2012-0935 Establishing an Effective Way to Co-culture the in-vitro Bovine Blastocysts from Different Methods With Their Uterine Endometrium Cells Zhang Yu1,2, Chen Liping1, Liang Hongbin2, Li Yajie1 Zhao Yaqi1, Yan Peng1, Yang Fei1, Ni Hemin1, Guo Yong1 (1College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206; 2Beijing Dairy Cattle Center, Beijing 100192) Abstract: In this study, it was initiated to establish an effective way to co-culture the bovine blastocysts and their uterine endometrium cells in vitro. Here, total 7 days in vitro fertilized (IVF) or 7 days parthenogenetic (PA) bovine blastocysts were put into the quadripuntal broad with 42,72 h uterine endometrium cells. The results showed that, after being co-cultured with the uterine endometrium cells for 48 h, the hatching rate of IVF blastocysts was significantly higher than that of PA group (40±5)% vs (25±5)%, P＜0.05), but after being co-cultured for 72 h, there was no significant differences of hatching rate between them (70?.66)% vs (65?)%, P0.05). In conclusion, these results suggested that, such kind of in vitro co-culture system between tllhose in vitro produced bovine blastocysts and the uterine endometrium cells, be a potential and effective way for further investigating the quality of bovine embryos. Key words: in vitro produced bovine blastocysts; uterine endometrium cell; in vitro co-culture 引言 妊娠建立和维持很大程度上依赖于一个完整的胚胎-母体对话系统。在反刍动物中,IFN-τ被证明是胚胎源的主