基于纳米自组装单分子膜的QCM传感分析研究毕业论文稿.docVIP

CHANGSHA UNIVERSITY OF SCIENCE TECHNOLOGY 毕业设计（论文） 题目：基于纳米自组装单分子膜的QCM传感分析研究 学生姓名： 张元元 学 号： 200729230210 班 级: 环工0702 专 业： 环境工程 指导教师： 曹 忠 2011 年 6 月 基于纳米自组装单分子膜的QCM传感分析研究 学生姓名： 张元元 学 号： 200729230210 班 级： 07-02 所在院(系): 化学与生物工程学院 指导教师： 曹 忠 完成日期: 2011年6月  基于纳米自组装单分子膜的QCM传感分析研究 摘要 本论文的实验是用L-半胱氨酸修饰电极表面，戊二醛共价交联法包被抗体，最后用纳米金标记二抗进行放大的方法检测蛋白，对压电免疫传感器进行了探索研究。首先在石英晶体金电极表面自组装单分子膜，，。L-半胱氨酸频率的士0Hz范围内变动，传感器容易再生。 关键词：纳米金；自组装单分子膜；QCM；c-myc  BASED ON NANO SELF-ASSEMBLED MONOLAYERS OF QCM SENSING ANALYSIS ABSTRACT Experiments of this paper is to use L-cysteine ??modified electrode surface, glutaraldehyde covalent crosslinking coating antibody, and finally labeled with gold nanoparticles to amplify the secondary antibody used to detect the protein on the piezoelectric immunosensor was explored study. First, the surface of piezoelectric quartz crystal gold electrode with thiol self-assembled layer of the cysteine monolayer, then assembled in the membrane layer of glutaraldehyde, through adsorption, the c-myc antibody fixed on the quartz crystal surface. By c-myc and c-myc protein antibody specific to c-myc protein in response to fixed on the crystal surface, finally, gold nanoparticles labeled secondary antibody GAM can significantly enlarge the antigen - antibody recognition signal. QCM on gold nanoparticles labeled with secondary antibody and a protein recognized by anti-continuous testing. The results show that L-cysteine ??or less constant before and after the change in the frequency range of 800Hz-1800Hz, Glutaraldehyde modification of the range before and after the change of frequency 1200Hz-3300Hz, c-myc antibody before and after a fixed frequency range 1000Hz-3000Hz, An anti-specific binding of antigen with the approximate frequency range before and after the 1000Hz-2800Hz, In 8.61 × 10-12 mol / L ~ 1.13 × 10-10 mol / L, between c-myc protein with increasing concentrations of