土肉桂之成分分析.docVIP

土肉桂之成分分析 Analysis of composition of Cinnamomum osmophloeum Kanehira 研究生：莊文宜 Chuang, Wen-Yi 指導教授：賴宏亮 博士 Lay, Horng-Liang 【摘要】 土肉桂﹙Cinnamomum osomphloeum Kanehira﹚為樟科﹙Lauraceae﹚常綠中喬木，為台灣特有種。本研究利用柱相層析﹙column chromatography﹚進行土肉桂成分的單離純化，從土肉桂葉部中分離得到24 種不同的化合物，經NMR 與MS 等光譜測定及文獻之比對，鑑定出其化合物成分分別為: β-sitosterol、ρ-hydroxybenzaldehyde、7-hydroxy-4′-methoxyisoflavone、benzoic acid、ρ-hydroxybenzoic acid、coumarin、4-hydroxycoumarin、cinnamic acid、cinnamaldehyde、ο-coumaric acid、trans- ρ- coumaric acid、cinnamyl alcohol、dihydrokaempferol、eugenol、linalool、linoleic acid、kaempferitrin、kaempferol-3- Ο-rhamnoside、kaempferol-7-rhamnoside、methyl gallate、palmitic acid、rutin、vanillin 及xylotetraose。進一步以ρ-hydroxybenzaldehyde、benzoic acid、ρ-hydroxybenzoicacid 4-hydroxy- coumarin、cinnamic acid、cinnamaldehyde、ο-coumaric acid、trans- ρ-coumaric acid、methyl gallate、rutin 以及vanillin 等11 種成分為指標成分，開發HPLC 分析方法，經確效試驗之驗證，得到安定且值得信賴之定量法，並進行土肉桂不同品種、不同部位及不同月份間之定量分析，探討其品質之差異性，經研究結果顯示，cinnamaldehyde 為土肉桂葉部與枝條之主成分，其中又以葉部含量較枝條為高，品系方面以台東土肉桂1 號之葉部與枝條成分含量較其他二品系為高。 關鍵字：香豆素、桂皮酸、桂皮醛、丁香酚、伽羅木醇。 【Abstract】 Indigenous cinnamon﹙Cinnamomum osmophloeum Kanehira﹚(Lauraceae), an endemic tree that grows in Taiwan, was used for isolation and characterization of its chemical components. The chemical constituents in the leaves of C. osmophloeum Kanehira were investigated with column chromatography. The structure of twenty-four compounds were establishd on the basis of NMR and MS analyses and data mining. These compounds include β-sitosterol, ρ-hydroxybenzaldehyde, 7-hydroxy-4′-methoxyisoflavone, benzoic acid, ρ-hydroxybenzoic acid, coumarin, 4-hydroxycoumarin, cinnamic acid, cinnamaldehyde, ο-coumaric acid, trans- ρ-coumaric acid, cinnamyl alcohol, dihydrokaempferol, eugenol, linalool, linoleic acid, kaempferitrin, kaempferol-3- Ο-rhamnoside, kaempferol-7-rhamnoside, methyl gallate, palmitic acid, rutin, vanillin and xylotetraose, respectively. Eleven compounds, including ρ-Hydroxybenzaldehyde, benzoic acid, ρ-hydroxybenzoic acid,