肠缺血再灌注损伤对leptin及orexin-A水平的影响.docVIP

肠缺血/再灌注损伤对leptin及orexin-A水平的影响 作者：林季1；颜光涛1；高晓宁2；廖杰3；郝秀华1；张凯1　　(解放军总医院1生化研究室，2小儿内科，3医学实验测试中心，北京 100853) 摘要：目的 研究肠缺血/再灌注(I/R)损伤对外周血及中枢分泌组织leptin及orexin-A水平的影响，探讨leptin及orexin-A在急性炎症反应中的作用。 方法 建立大鼠肠I/R损伤模型，设立缺血60 min后不同时间的再灌注损伤组。采用放射免疫分析法测量血清及脂肪组织leptin、血浆及下丘脑orexin-A蛋白水平的变化，并用逆转录-聚合酶链式反应法检测脂肪组织leptin、下丘脑orexin-A mRNA表达水平的变化。结果 与损伤前自身对照相比，缺血60 min/再灌注30 min组(I60’R30’)血清leptin显著降低，I60’R360’组显著增高。与损伤后假手术组(sham)相比，I60’R360’组血清Leptin水平显著增高。与损伤后sham组相比，I60’R30’、I60’R90’组脂肪leptin蛋白水平显著降低，I60’R360’组显著增高。与损伤后Sham组相比，I60’R30’、I60’R240’、I60’R360’组脂肪leptin mRNA表达显著增高，I60’R150’组显著降低。各组损伤前、后的血浆及下丘脑orexin-A蛋白水平无显著性差异。与损伤后sham组相比，I60’R30’、I60’R90’组下丘脑orexin-A mRNA表达逐步降低，I60’R150’组最低，I60’R240’、I60’R360’组逐步回升但仍低于sham组。结论 Leptin及orexin-A对肠I/R损伤的应答具有时间依赖效应，leptin比orexin-A的应答较迅速，两者可能在急性炎症反应的能量代谢障碍中发挥一定的作用。 关键词：缺血；肠；再灌注损伤；leptin；orexin-A；脂肪；下丘脑；放射免疫分析；逆转录-聚合酶链式反应 中图分类号：R392.32??文献标识码：A??文章编号：1673-4254(2006)06-0719-06 Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levelsLIN Ji1; YAN Guang-tao1; GAO Xiao-ning2; LIAO Jie3; HAO Xiu-hua2; ZHANG Kai11Research Laboratory of Biochemistry, 2Department of Pediatrics, 3Medical and Laboratory Analysis Center, General Hospital of PLA, Beijing 100853, ChinaAbstract: Objective To explore the effect of intestinal ischemia/reperfusion (I/R) injury on leptin and orexin-A levels in peripheral blood and central secretory tissues, and investigate the roles of leptin and orexin-A in acute inflammatory responses. Methods An intestinal I/R injury rat model was established, and the rats were grouped according to duration of the reperfusion time following a 60-min ischemia. Radioimmunoassay was used to examine the protein levels of leptin in the serum and adipose tissue, and the protein levels of orexin-A in the plasma and hypothalamus. Reverse transcriptase- polymerase chain reaction was also performed to detect the mRNA expressions of adipose leptin and hypothalamus orexin-A. Results Compared with that before injury, serum leptin