慢速冷冻和玻璃化冷冻对人类卵巢组织中卵泡形态的影响.DOCVIP

临床医学论文-慢速冷冻和玻璃化冷冻对人类卵巢组织中卵泡形态的影响 ?????????????? 作者：彭艳， 于丛一， 李 予， 麦美琪， 冯淑英， 杨冬梓， 张清学??? 【摘要】? 　　【目的】 比较慢速冷冻、麦管玻璃化冷冻和尼龙网玻璃化冷冻对人类卵巢组织中卵泡形态的影响。【方法】 16例人卵巢组织切成薄片随机分配到新鲜卵巢组(A组)、麦管玻璃化冷冻组（B组）、尼龙网玻璃化冷冻组（C组）和慢速冷冻组（D组）后行组织学和电镜检查。【结果】 A、B、C、D组中形态正常的原始卵泡比例分别为72.5%±8.4%、65.6%±12.8%、 66.1%±11.1%、48.4%±13.3%；形态正常的初级卵泡比例分别为62.0%±13.9%、58.1%±7.9%、59.0%±16.2%、37.0%±14.0%。D组中形态正常的原始卵泡比例和初级卵泡比例均明显低于A、B、C组。B、C组形态正常的原始卵泡比例和初级卵泡比例，与A组相比无统计学差异。B、C组中形态正常的原始卵泡超微结构无明显改变，D组中形态正常的原始卵泡超微结构存在一定程度的改变。【结论】 玻璃化冷冻是人类卵巢组织较适宜的冷冻保存方法。 【关键词】? 卵巢组织; 慢速冷冻; 玻璃化冷冻； 卵泡 ??? Abstract:【Objective】 To compare the effect of slow cooling with vitrification to get a more effective procedure for human ovarian tissue cryopreservation.【Methods】 Ovarian biopsies from 16 patients were cut into ovarian pieces (OPs) of 1mm thickness which randomly distributed into fresh OPs (Group A), conventional-straw vitrificated OPs (Group B), vitrificated OPs using nylon mesh (Group C) and slow cooled OPs (Group D) treatment Groups. Histological and ultrastructural observation of OPs was performed after cryopreservation.【Results】 The proportions of morphologically normal primordial and primary follicles from Group A, Group B, Group C, and Group D were 72.5%±8.4% and 62.0%±13.9%, 65.6%±12.8% and 58.1%±7.9%, 66.1%±11.1% and 59.0%±16.2%, 48.4%?3.3% and 37.0%?4.0%, respectively. The proportions of morphologically normal primordial and primary follicles from Group D were lower than those from Group A, Group B, and Group C. The difference in proportions of morphologically normal primordial follicles among Group A, Group B, and Group C was not significant, so were the proportions of morphologically normal primary follicles. The ultrastructural studies showed that in primordial follicles considered normal at histological analysis, there are no difference among Group A, Group B, and Group C. While there were a few abnormalities in Group D.【Conclusion】 Vitrification is more favorable than slow cooling in human ovarian cryopreservation. ??? Ke