IL23和IL17在结直肠癌患者中的表达及意义.pdfVIP

580 中国肿瘤临床2014年第41卷第9期 ChinJClinOncol2014,Vol.41,No.9 IL-23和IL-17在结直肠癌患者中的表达及意义 王建升 吕元鹏 孟军涛 宋津晓 张立志 帖彦清 检测结直肠癌患者外周血和肿瘤组织中IL-23、IL-17的含量，探讨其与临床病理特征的相关性。 24例 摘要 目的： 方法： 对照组和56例结直肠癌患者组。用ELISA法检测患者和对照组外周血及患者正常黏膜组织与肿瘤组织培养液中的IL-23、 IL-17水平。 结直肠癌患者外周血和肿瘤组织培养液中IL-23和IL-17水平分别高于相应的对照组（P0.01）和正常黏膜 结果： 组（P0.05）。结直肠癌患者外周血中IL-17水平与淋巴结转移情况及TNM分期相关（P0.05），但IL-23水平与患者各项临床病 理参数无相关性（P0.05）。肿瘤组织培养液中IL-17水平与肿瘤分化程度、淋巴结转移情况及TNM分期相关（P0.05），而IL-23 水平仅与TNM分期相关（P0.05）。 IL-23和IL-17可能参与了结直肠癌进展的免疫病理过程。 结论： 关键词 结直肠癌 IL-23 IL-17 doi:10.3969/j.issn.1000-8179 Expression and clinical significance of IL-23 and IL-17 in patients with colorectal cancer Jiansheng WANG, Yuanpeng LV, Juntao MENG, Jinxiao SONG, Lizhi ZHANG, Yanqing TIE Correspondence to: Jiansheng WANG; E-mail: wjsxjr@126.com Department of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China. Abstract Objective: This study was conducted to investigate the levels of IL-23 and IL-17 in sera and tumor tissues and to ana- lyze their association with clinico-pathological parameters in patients with colorectal cancer (CRC). Methods: A total of 24 healthy sub- jects and 56 patients with CRC were recruited in this study. Enzyme-linked immunosorbent assay was performed to measure the concen- trations of IL-23 and IL-17 in the sera of CRC patients and healthy controls and in cultured normal tissues and tumor tissues from CRC patients. Results: The levels of serum IL-23 and IL-17 were significantly higher in CRC patients than in healthy controls (P0.01). The supernatant levels of IL-23 and IL-17 in tumor tissues were significantly higher than in normal tissues (P0.