黄芪注射液对内皮细胞损伤的保护效应.docVIP

黄芪注射液对内皮细胞损伤的保护效应 作者：杨富国 董果雄 张社华 【关键词】 黄芪注射液;内皮细胞;血管;细胞低氧;细胞培养技术 　　［摘要］目的 了解黄芪注射液对内皮细胞损伤的保护作用及其机制。方法 人脐静脉内皮细胞原代培养，传至3代后，分为3组。对照组:常规培养;低氧复氧组:先低氧处理1 h，再复氧1 h;药物干预组:在培养液中加入终浓度分别为5、10、20、40、60 mg/L的黄芪注射液，12 h后进行低氧复氧处理。测定各组细胞上清液中乳酸脱氢酶（LDH）活性、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，检测内皮细胞台盼蓝摄取率。结果 低氧复氧组与对照组比较，细胞培养液中LDH活性、MDA含量及内皮细胞台盼蓝摄取率升高，SOD活性减低，差异均有显著性（ F=34.97～129.98，q=14.43～27.11，P lt;0.01）。药物干预组与低氧复氧组比较，细胞培养液中LDH活性、MDA含量及内皮细胞台盼蓝摄取率降低，SOD活性升高，差异均有显著性（ q=2.91～26.61，P lt;0.05、0.01）。黄芪注射液预处理浓度为40 mg/L时LDH活性、SOD活性、MDA含量与对照组比较差异无显著性（ q=0.49～ 0.94 ，Pgt; 0.05）;黄芪注射液预处理浓度60 mg/L与40 mg/L相比，LDH活性、SOD活性、MDA含量变化均不明显。结论 低氧复氧过程造成内皮细胞脂质过氧化损伤，细胞死亡率增加。黄芪注射液可能通过抗脂质过氧化，对低氧复氧内皮细胞产生保护作用。 　　［关键词］ 黄芪注射液;内皮细胞;血管;细胞低氧;细胞培养技术 　　［ABSTRACT］ObjectiveTo investigate the protective effects of Astragalus Membranaceus Injection （AMI） on endothelial cell injury and its mechanism. MethodsHuman umbilical venous endothelial cells （HUVECs） were primarily cultured， passed down to the third generation， and then divided into three groups. Control group: the cells were conventionally cultured; hypoxia/re-oxygenation group: the cells were treated with hypoxia （1 h） and then re-oxygenation （1 h）; AMI group: different doses of AMI （5， 10， 20， 40， 60 mg/L） were added into the culture medium， and hypoxia/re-oxygenation was managed 12 hours later. The activity of LDH and superoxide dismutase （SOD）， content of malondialdehyde （MDA） in cell medium were determined. The up-take rate of trypan-blue in each group was detected. Results In hypoxia/reoxygenation group， the activity of LDH， the con-tent of MDA， and the up-take rate of trypan-blue were higher， and the activity of SOD was lower than the control group， the differences were significant （ F=34.97-129.98， q=14.43-27.11， P lt;0.01）. In AMI group， the activity of LDH， the content of MDA ，and the up-take rate of trypan-blue decreased， and SOD activity increased as compared with hypoxia/reoxygenation group， the differences were significant