浙江省灰飞虱抗药性及其与体内共生菌的关系.doc

杭州师范大学硕士学位论文  浙江省灰飞虱抗药性及其与体内共生菌的关系  间越长，灰飞虱对这三种农药的敏感性越强，即敏感性顺序：3 d2 d1 d。 关键词：灰飞虱，抗药性，杀虫剂配方筛选，类酵母共生菌，共生菌形态特征，冷冻切片， 显微观察 II 杭州师范大学硕士学位论文  浙江省灰飞虱抗药性及其与体内共生菌的关系  Abstract The small brown planthopper (Laodelphax striatellu) is one of the major pest of rice in Jiangzhe area, which is not only sucks rice juice directly, but also transmits virus disease and cause reduction of rice yield. The main method of the small brown planthopper (SBPH) control is utilization of chemical insecticides, and imidacloprid, chlorpyrifos and so on, which are the common insecticides. While recently there are evidences showing that these two insecticides have been the trend of resistance. In this study, for preventing the SBPH effectively and delaying their insecticide resistance, the resistance of SBPH, collected from Jiaxing, Ningbo, Huzhou, Jinhua, Wenzhou, Hangzhou in Zhejiang province, to insecticides, imidacloprid, fipronil and chlorpyriphos, was studied, then the good effect formula of complex agent were selected on field-controlling. In order to study the relationship of the yeast-like endosymbiote (YLES) in abdomen fat body of SBPH and resistance of SBPH, morphologyical characteristics of YLES in SBPH were studied by the cryostat microtome method in combination with the photomicrography; the sensitivity of SBPH with different number YLES by treatment of 35℃, to insecticides were studied, while the relationship between sensitivity to insecticides and the YLES in the body of the SBPH is initially revealed. The results are as following: 1. The resistance of the SBPH population, collected from different locations in Zhejiang province, to insecticides, imidacloprid, fipronil and chlorpyriphos, was studied. Results indicated that in the rice field of Zhejiang Province the SBPH had the resistance to imidacloprid and the higher resistance of Jinhua, Hangzhou, Ningbo SBPH population were up to 14.7, 11.3 and 10 times compared with susceptible population, and all reached to stander of med