HPLC法测定龙芽楤木药材中齐墩果酸的含量.pdfVIP

·46· Chinese Journal of Information on TCM Jan.2010 Vol.17 No.1 HPLC法测定龙芽楤木药材中齐墩果酸的含量 谭朝阳,袁宏佳 (湖南中医药大学药学院,湖南 长沙 410208) 摘要：目的 建立 HPLC 法测定龙芽楤木药材中齐墩果酸的含量测定方法。方法 采用 Kromasil C18 色谱柱 (250 mm×4.6 mm,5 µm),流动相：乙腈-水-磷酸(80∶20∶0.42),检测波长：208 nm。结果 齐墩果酸在1.164～5.820 µg 范围内具有良好的线性关系,r ＝0.999 9,平均加样回收率为99.69%,RSD＝1.76%(n ＝5)。结论 该方法简单、准确, 重复性好,可作为龙芽楤木药材中齐墩果酸的含量测定方法。 关键词：龙芽楤木；齐墩果酸；高效液相色谱法；含量测定 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2010)01-0046-02 Determination of Oleanolic Acid in Cortex Aralia Elatae by HPLC TAN Zhao-yang, YUAN Hong-jia (Pharmacy College, Hunan University of TCM, Changsha 410208, China) Abstract ：Objective To establish a method for determination of oleanolic acid in Cortex Aralia Elatae by HPLC. Methods The determination was carried out with Kromasil C column (250 mm ×4.6 mm, 5 µm), 18 using acetonitrile-water-phosphoric acid (80 ∶20 ∶0.42) as the mobile phase and detected at the wavelength of 208 nm. Results Oleanolic acid showed a good linear relationship at the range of 1.164～5.820 µg, r ＝ 0.999 9. The average recovery was 99.70% and RSD was 1.76% (n ＝5). Conclusion The method was simple and accurate with good reproducibility, and can be used to determine the content of oleanolic acid in Cortex Aralia Elatae. Key words ：Cortex Aralia Elatae；oleanolic acid；HPLC；content determination 龙芽楤木,别名辽东楤木、刺龙牙,为五加科植物龙芽楤木 Aralia elata (Miq.)Seem.的干燥根皮及茎皮,产于吉林、辽 宁、黑龙江等地,具有益气补肾、祛风利湿、活血止痛之功效, A 龙芽楤木皂苷是其主要化学成分之一。本试验采用高效液相色 谱(HPLC)法对龙芽楤木药材中苷元齐墩果酸的含量进行了测 定,取得了满意的结果。