解氨酶活性及其基因表达的影响.pdfVIP

※生物工程 食品科学 2014, Vol.35, No.11 135 5-氨基乙酰丙酸对苹果幼果多酚含量、苯丙氨酸 解氨酶活性及其基因表达的影响 * 陈 磊，郭玉蓉，刘永峰 ，米瑞芳，窦 娇 （陕西师范大学食品工程与营养科学学院，陕西西安 710062 ） 摘  要：探究5- 氨基乙酰丙酸（aminolevulinic acid ，ALA ）处理对苹果幼果酚类物质、苯丙氨酸解氨酶 （phenylalanin ammonia-lyase ，PAL ）活性及其基因表达量的影响。在苹果疏果期前，利用不同质量浓度ALA处理 苹果幼果，采用紫外分光光度法测定ALA处理后12 d内苹果多酚和PAL活性，利用荧光定量法测定苹果幼果PAL基 因表达量。结果表明：ALA处理质量浓度在300 mg/L 以内，随着质量浓度提高，苹果多酚含量、PAL酶活性及其基 因表达量均升高，到400 mg/L时各项指标均表现出下降趋势；相同质量浓度ALA处理苹果幼果后，幼果的上述3 项 指标均随时间延长而明显提高，在第9天时达到最高值，12 d后开始下降。为提高苹果疏果多酚含量，实现疏果的 最大利用率，生产中需要选择适宜的ALA处理浓度和时间采集幼果。 关键词：苹果幼果；多酚；苯丙氨酸解氨酶；基因表达 Effect of 5-Aminolevulinic Acid on Polyphenol Content, PAL Activity and PAL Gene Expression in Young Apple Fruits * CHEN Lei, GUO Yu-rong, LIU Yong-feng , MI Rui-fang, DOU Jiao (College of Food Engineering and Nutritional Sciences, Shaanxi Normal University, Xi ’an 710062, China) Abstract: The purpose of this study was to find out the effect of 5-aminolevulinic acid (ALA) application on phenolic substances and phenylalanine ammonia lyase (PAL) activity and PAL gene expression in young apple fruits. Apple fruits were treated with various concentrations of ALA before thinning, and then over a subsequent period of 12 days, total phenolic contents and PAL activity were measured by UV spectrophotometry and the expression of PAL gene by fluorescent quantitative real-time polymerase chain reaction (RT-qPCR). The experiment results indicated that as the ALA concentration increased up to 300 mg/L, the PAL activity, total phenolic content and PAL gene expression levels all showed an increasing trend, while the opposite pattern was observed for these three par