闽西南黑兔一般抗病特性的研究_陈冬金.docVIP

中国农学通报  2014,30(2):26-29 Chinese Agricultural Science Bulletin 闽西南黑兔一般抗病特性的研究 陈冬金 1，陈岩锋 1，谢喜平 1，孙世坤 1，桑 雷 1，丁晓红 2 1 摘 要：为了探讨闽西南黑兔品种的抗病特性，通过测定其体液免疫指标和细胞免疫指标，全面系统地评 价闽西南黑兔的免疫力，间接反映其非特异性抗病特性。本研究对 90 日龄的闽西南黑兔（24 只，雌雄各 半）测定其血清中的免疫球蛋白 IgG、IgM、IgA 含量，补体 C3、C4 含量，以及外周血中淋巴细胞转化力和 中性粒细胞吞噬力。结果表明：闽西南黑兔的免疫球蛋白 IgG、IgM、IgA 含量分别为 2031.22、96.78、 10.94 mg/L，补 体 C3、C4 含 量 为 55.15、17.89 mg/L，淋 巴 细 胞 刺 激 指 数 为 1.50 和 中 性 粒 细 胞 吞 噬 率 为 58.39%，中性粒细胞吞噬指数为 2.22，且雌雄差异不显著(P0.05)。本试验测定的免疫指标为闽西南黑 兔一般抗病力的选择以及非特异性抗病育种提供依据。 关键词：闽西南黑兔；免疫球蛋白；补体；淋巴细胞转化率；中性粒细胞吞噬率 中图分类号：S829.1 文献标志码：A 论文编号：2013-2777  Study of General Resistance Characteristics on Minxinan Black Rabbit Chen Dongjin1, Chen Yanfeng1, Xie Xiping1, Sun Shikun1, Sanglei1, Ding Xiaohong2 (1Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013; 2 Abstract: In order to investigate the resistance characteristics of Minxinan Black Rabbit, the humoral immunity and cellular immunity indicators were measured, which could evaluate Minxinan Black Rabbit immunity comprehensively and systematacially, and that also indirectly reflect the non-specific resistance characteristics. In this paper, the content of immunoglobulin IgG, IgM, IgA and complement C3, C4 in Minxinan Black Rabbit’s (90 days, 12 male and 12 female) serum were determinated, the change of the peripheral blood lymphocyte transformation and the phagocytosis ability of neutrophil were tested. The results showed that,the content of immunoglobulin IgG, IgM, IgA on Minxinan Black Rabbit were 2031.22 mg/L, 96.78 mg/L, 10.94 mg/L, complement C3, C4 were 55.15mg/L, 17.89 mg/L, lymphocyte stimulation index was 1.50 ，neutrophil phagocytic rate was 58.39% and neutrophil phagocytic index is 2.22. IgG, IgM, IgA , C3, C4 has no significant difference between sex (P0.05). The immune indexes may provide a reference in general resistance selection and nonspecific resistance breeding. Key words: rate; neutrophil phagocytic rate 0