低频电刺激改善脑梗死大鼠神经功能障碍的机制研究①.pdfVIP

中国康复理论与实践2014年4月第20卷第4期ChinJRehabilTheoryPract,Apr.,2014,Vol.20,No.4 - 301 - DOI: 10.3969/j.issn. 1006-9771.2014.04.001 ·专题 · 低频电刺激改善脑梗死大鼠神经功能障碍的机制研究① 1 2 2 2 何志承 ，杨万章 ，向云 ，王维 [摘要] 目的 探讨低频电刺激(LFS)对脑梗死大鼠神经功能和梗死侧脑组织神经干细胞(NSC)增殖、血管再生的影响。方法 制作大鼠永久性大脑中动脉梗死模型(MCAO) ，随机分为假手术组、对照组和实验组，每组又分为7 d 和14 d 两个亚组，每个亚组 12 只。术后2 d 开始进行LFS 治疗。采用大鼠神经功能缺损评分(NSS)评估大鼠神经功能缺损程度，运用免疫荧光染色检测大鼠梗 死侧大脑侧脑室的室管膜下层(SVZ)的5-溴尿嘧啶脱氧核糖核苷酸(BrdU) ，应用酶联免疫吸附测定法(ELISA)检测大鼠梗死侧大脑 的基质细胞衍生因子- 1(SDF- 1) 、血管内皮生长因子(VEGF)含量。结果 LFS 治疗后14 d ，实验组大鼠NSS 评分明显低于对照组和 假手术组(P0.01) ；LFS 治疗后各时间点实验组大鼠梗死侧大脑的SDF- 1、BrdU 和VEGF 水平均较对照组有不同程度的上调(P 0.01) 。结论 LFS 能改善脑梗死大鼠功能障碍，其可能机制是通过激活SDF-1/CXCR4 轴，进而发挥促进NSC 增殖和血管再生作 用。 [关键词] 低频电刺激；基质细胞衍生因子-1；5-溴尿嘧啶脱氧核糖核苷酸；血管内皮生长因子；大鼠 Effect of Low Frequency Stimulation on Neural Dysfunction in Rats with Cerebral Infarction HE Zhi- cheng, YANG Wan- zhang, XIANG Yun, et al. Graduate School, Guangzhou Medical University, Guangzhou 510182, Guangdong, China Abstract: Objective To investigate the effects of low frequency stimulation (LFS) on proliferation and angiogenesis of neural stem cells (NSC) in cerebral infarction side in rats. Methods The rats with permanent middle cerebral artery occlusion (MCAO) were randomly divided into sham-operation group, control group and LFS group, each group was divided into 7 days and 14 days subgroups with 12 rats in each subgroup. LFS therapy was started 2 days after operation. The degree of nerve function defect was evaluated with Neurological Severi- ty Score (NSS), and the 5-bromodeoxy-dine (BrdU) positive cells in the subventricular zone (SVZ) of cerebral infarction sid