基础研究菝葜治疗慢性盆腔炎有效部位的药效学筛选研究.pdfVIP

J South Med Univ, 2013, 33(1): 145-149 doi 10.3969/j.issn. 1673-4254.2013.01.33 ·145 · 基础研究 菝菝葜治疗慢性盆腔炎有效部位的药效学筛选研究葜治疗慢性盆腔炎有效部位的药效学筛选研究 马 云，罗艳琴，宋路遥，秦 飞，侯连兵 南方医科大学南方医院药学部，广东 广州 510515 摘要：目的 通过对菝葜各化学部位的药效学筛选，确定其治疗慢性盆腔炎疾病（CPID）的有效部位，为菝葜制剂的二次开发提 供科学依据。方法 采用苯酚胶浆注入大鼠子宫造成大鼠CPID模型，观察菝葜各化学部位对CPID模型大鼠的治疗作用；采用 二甲苯致小鼠耳肿胀法和大鼠棉球肉芽肿法，探讨菝葜各化学部位的抗炎作用。结果菝葜乙酸乙酯部位的高剂量组能显著降 低CPID模型大鼠的子宫炎症反应，对小鼠耳肿胀和大鼠棉球肉芽肿的抗炎作用与模型对照组比较均有显著性差异（P0.01）； 中剂量组也能明显改善炎症程度。总体抗炎作用优于总提液组。正丁醇部位作用不明显。结论菝葜的乙酸乙酯部位为菝葜 抗炎及抗慢性盆腔炎的有效部位。 关键词：菝葜；有效部位；慢性盆腔炎；抗炎；筛选 Pharmaceutical screening of the effective fraction from Smilax for treatment of chronic pelvic inflammatory disease MA Yun, LUO Yanqin, SONG Luyao, QIN Fei, HOU Lianbing Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To determine the effective fraction of Smilax for treatment of chronic pelvic inflammatory disease (CPID) by pharmacodynamic screening as the basis for further development of sarsaparilla preparations. Methods The chemical fractions of Smilax were administered intragastrically in rat models of CPID induced by injecting phenol mucilage into the uterus to observe the therapeutic effects. The anti-inflammatory effects of different extract fractions from Smilax were tested in mice with xylene-induced ear edema and in rats with cotton-ball-induced granuloma. Results High-dose ethyl acetate extract of Smilax could obviously inhibit uterus inflammation in rats with CPID, showing also strong anti-inflammatory effects against ear edema in mice and granuloma in rats (P0.01). The moderate dose of ethyl acetate extract also obviously ameliorated the inflammation. Both the ethyl acetate extract fraction and the total extract fraction of Smilax showed anti-inflammatory effects, while the former produced strong effects while the lat