猪PRLR和RBP4基因多态性与产仔性能的关系.pdfVIP

HEREDITAS (Beijing) 2009 1 , 31(1): 63―68 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2009.00063 猪PRLR 和RBP4 基因多态性与产仔性能的关系 孙延晓, 曾勇庆, 唐辉, 樊新忠, 陈其美, 李华, 钱源, 宋一萍 , 271018 采用 PCR-RFLP 方法, 对莱芜黑猪、鲁莱黑猪、里岔黑猪、鲁烟白猪、新沂蒙黑猪 5 个山东地方/培育 猪种和大约克夏、长白、杜洛克3 个引进猪种共8 个猪种323 头繁殖母猪进行PRLR 和RBP4 基因的多态性检 测, 并采用最小二乘法分析其对产仔数影响的遗传效应。结果表明: 两个基因位点在 8 个猪种的测定群体中均 存在多态性, 但山东地方/培育猪种与引进猪种间在基因型频率上存在较大差异。PRLR 和RBP4 基因对产仔数 性状有显著影响(P 0.05), AA 均为优良基因型。对于PRLR 基因, 山东地方/培育猪种内AA 基因型母猪的总产 仔数和活产仔数比BB 基因型母猪平均多产 1.03 头和0.89 头, 引进猪种中AA 基因型母猪比BB 基因型母猪平 均多产分别为 1.26 头和1.11 头。对于RBP4 基因, 山东地方/培育猪种内AA 基因型母猪的总产仔数和活产仔数 比BB 基因型母猪平均多产0.59 头和0.51 头, 引进猪种中AA 基因型母猪比BB 基因型母猪平均多产分别为0.72 头和0.64 头。 猪; PRLR 基因; RBP4 基因; 多态性; 产仔数 Relationship of genetic polymorphism of PRLR and RBP4 genes with litter size traits in pig SUN Yan-Xiao, ZENG Yong-Qing, TANG Hui, FAN Xin-Zhong, CHEN Qi-Mei, LI Hua, QIAN Yuan, SONG Yi-Ping College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China Abstract: The polymorphism of prolactin receptor (PRLR) and retinol-binding protein 4 (RBP4) genes was detected by PCR-RFLP method, and the effects of PRLR and RBP4 genes on litter size traits in pig were analyzed by the least square analysis. Data of total number born (TNB) and number born alive (NBA) from 323 sows, including five Shandong local pig breeds and three foreign pig breeds, were collected. The results showed that the polymorphic sites of both PRLR and RBP4 genes were found in all populations tested. The genotype distribution, however, revealed great differences between Shan- dong local pig breeds and foreign pig breeds. The effects of genotypes on TNB and NBA were significant (P0.05). The homozygote AA was the most prolific genotype. For PRLR ge