猪核糖体蛋白基因RPLP0的cDNA全长、染色体定位和多态性分析.pdfVIP

Journal of Genetics and Genomics (Formerly Acta Genetica Sinica) February 2007, 34(2): 104-108 Research Article Characterization of the Full-length cDNA, Chromosomal Lo- calization, and Polymorphism of the Porcine RPLP0 Gene 1, 2 1 1 1 1,① Xiao Wu , Shulin Yang , Zhengmao Zhu , Shutang Feng , Kui Li 1. Department of Gene and Cell Engineering , Institute of Animal Science , Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. Department of Animal Genetics and Breeding, School of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China Abstract: RPLP0 gene encodes the acidic ribosomal phosphoprotein large P0 subunit, which is a component of the 60S subunit. The full-length cDNA sequence of porcine RPLP0 was obtained from skeletal muscle of fetal pig cDNA library and deposited in GenBank. The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homo- logues. A C/A single nucleotide substitution in exon 5 was detected as Csp6 Ⅰpolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency diversity among Tongcheng, Xiaomeishan, Yushan, Large White, Landrace, and Duroc breeds. Analyses of somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel showed that the RPLP0 gene was mapped to SSC 14q22-q24 and was closely linked to locus SW1321 (25 cR, LOD = 14.54). Keywords: RPLP0; porcine; IMpRH; SCHP; polymorphisms Ribosomes, the organelles that catalyze protein ribosomal phosphoproteins P1 and P2. The P0 protein synthesis, consist of a small 40S subunit and a large can interact with P1 and P2 to form a pentameric 60S subunit. These subu