植物基因组DNase I超敏感位点的研究进展.pdfVIP

HEREDITAS (Beijing) 2013 7 , 35(7): 867―874 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2013.00867 植物基因组DNase I 超敏感位点的研究进展 张韬, 杨足君 , 610054 真核生物的基因表达与调控是一系列顺式作用元件(cis-acting element)与反式作用因子(trans-acting fac- tor)相互作用的结果。顺式作用元件本身不编码蛋白, 只是基因组中一些特定的区域, 它们需与反式作用因子结 合才能发挥其生理作用。目前已知, 顺式作用元件往往与染色质的结构密切相关, 能与反式作用因子结合发挥 生物学功能的顺式作用元件, 通常存在于脱氧核糖核酸酶I 超敏感位点 (DNase I hypersensitive site) 的内部或 周围。随着越来越多的植物基因组测序的进展, 大规模高通量鉴定植物基因组的顺式作用元件正在受到关注, 通过鉴定脱氧核糖核酸酶I 超敏感位点可以有效确定顺式作用元件的富集区域, 将对功能基因组学研究提供的 数据支持。文章综述了近期高通量鉴定和分析植物基因组脱氧核糖核酸酶 I 超敏感位点研究进展。 基因表达调控; 脱氧核糖核酸酶I 超敏感位点; 植物基因组 Progress on identification and analysis of DNase I hypersensitive sites in plant genomes ZHANG Tao, YANG Zu-Jun School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China Abstract: Eukaryotes’s gene expression and regulation relies on the interaction of their cis-acting elements and trans-acting factors. The cis-elements are specific DNA sequences in the genome and frequently located in the untranslated regions. The trans-acting factors are usually considered to be proteins that bind to the cis-acting sequences to regulate gene expression. It is well known that the cis-elements are always associated with DNase I hypersensitive site, which is a signa- ture of open chromatin. The identification of all the functional cis-element using high-throughput method in plant genome has not been initiated in plant genomes. With the rapid achievement of genomics studies, an increasing number of plant genomes have been sequenced. Genome-wide identification