60Coγ射线对龙牧803处理后M4代与Beza87杂花苜蓿杂交的研究.pdfVIP

60Co γ射线对龙牧803 处理后M4 代 与Beza87 杂花苜蓿杂交的研究 * 刘凤歧张月学 唐凤兰刘杰淋韩微波 （黑龙江省农业科学院草业研究所，哈尔滨，150086） 摘 要：通过60Co γ射线辐射诱变龙牧803 干种子，利用辐射与杂交相结合的方法进行苜蓿 人工杂交，结果表明60Co γ射线处理的龙牧803 M4 代不论做父本还是母本与Beza87 杂交 与对照相比均取得了较高的结实率，正交的结实率为25.37% 、反交的结实率为50%，对照 组的正交结实率为6.12%、反交的结实率为9.33% 。经方差分析结果表明，60Co γ射线辐射 处理的龙牧803 M4 代与未辐射的龙牧803 做亲本杂交后代的结实率表现出了极显著的差异 性，相同亲本正反交后代的结实率差异显著，重复之间差异不显著，说明60Co γ射线在苜蓿 杂交育种中对苜蓿杂交的结实率有显著影响。 关键词：60Co γ射线；辐射诱变；龙牧803；Beza87 ；杂交 Influence of alfalfa hybridization on Setting Percentage with 60Co γ-rays LIU Feng-qi ZAHNG Yue-xue TANG Feng-lan LIU Jie-lin HAN Wei-bo （Institute of Pratacultural Sciences，Heilongjiang Academy of Agricultural Sciences,Harbin,150086 ） Abstract : Combining with radiation and hybridization to make the artificial hybridization with Longmu No.803 dry seeds radiated by 60Co γ-ray and Beza87.Results indicates that Longmu No.803 seeds irradiated by 60Co γ-ray 60 are all acquired higher propagation coefficient than the control, whether they are male parent or female parent.The propagation coefficient of orthogonal seed is 25.3%, and the anti-seed-setting-rate is 50%. In the control, the propagation coefficient of orthogonal seed is 6.12%, and the anti-seed-setting-rate is 9.33%. The results of the analysis of variance shows that it is extreme distinguished variability in hybrid’s propagation coefficient between the irradiated and the non-irradiated seeds as the parents. It is distinguished variability among the reciprocal cross offspring of the same parent, but it is not variability among the duplication. It illustrates that the 60Co γ-ray can improve set propagation coefficient of hybrids obviously in alfalfa hybridization. Key words : 60Co γ-rays ；radiation mutation ；Longmu No.803 ；Beza