不同填闲模式对黄瓜根际土壤酶活性及细菌群落的影响.pdfVIP

网络出版时间：2014-10-10 10:03 网络出版地址：/kcms/doi/10.13287/j.1001-9332001.html 不同填闲模式对黄瓜根际土壤酶活性及细菌群落的影响 李 敏 吴凤芝* （东北农业大学园艺学院，哈尔滨150030) 摘 要 以温室连作3年黄瓜土壤为研究对象, 以黄瓜为主栽作物, 以青葱、小麦、油菜为不同季节填闲作物 设置盆栽试验,采用常规化学方法、PCR-DGGE及qPCR技术,研究不同填闲模式对黄瓜土壤酶活性及细菌群 落的影响.结果表明: 随着种植茬次的增加,填闲小麦处理的土壤脲酶、中性磷酸酶及转化酶活性均显著高于 填闲青葱和油菜处理, 同时油菜处理显著高于青葱处理(P0.05);不同填闲模式间黄瓜根际土壤细菌群落结构 不同,冬季填闲青葱和夏季填闲小麦处理维持了相对较高的多样性指数;qPCR检测结果表明: 随着种植茬次 的增加,小麦处理的土壤细菌数量显著高于青葱和油菜处理(P0.05).综上,不同填闲模式对土壤酶活性和细 菌群落均产生一定影响,改变了土壤环境,其中夏季填闲小麦能保持相对较高的土壤酶活性、土壤细菌群落结 构多样性及细菌数量. 关键词 填闲模式 黄瓜 土壤酶活性 细菌群落 文章编号 中图分类号 Q5 文献标识码 A Effects of different catch modes on soil enzyme activities and bacterial community in the rhizosphere of cucumber. LI Min, WU Feng-zhi (College of Horticulture, Northeast Agricultural University, Harbin 150030, China) Abstract: Effects of different catch modes on soil enzyme activities and bacterial community in the rhizosphere of cucumber (Cucumis sativus) were analyzed by conventional chemical method, PCR-denaturing gradient gel electrophoresis (DGGE) and real-time PCR methods. Pot experiment was carried out in the greenhouse for three consecutive years with cucumber as the main crop, and scallion (Allium fistulosum), wheat (Triticum aestivum) and oilseed rape (Brassica campestri) as catch crops. Results showed that, with the increase of crop planting times, soil urease, neutral phosphatase and invertase activities in the wheat treatment were significantly higher than in the scallion and oilseed rape treatments (P0.05), and these