SARS冠状病毒N蛋白激活hfgl2凝血酶原酶基因的研究.pdfVIP

第 21 卷 4 期 中 国 病 毒 学 21 （4 ）：319-323 2006 年 7 月 VIROLOGICA SINICA July 2006 SARS 冠状病毒N 蛋白激活hfgl2 凝血酶原酶基因的研究* 1 2 1 2 1 1** 姚华宁 ，王战会 ，韩梅芳 ，侯金林 ，罗小平 ，宁 琴 （1.华中科技大学同济医学院附属同济医院感染免疫研究室感染科，湖北武汉 430030 ；2 ．南方医院感染内科，广东广州 510515 ） Nucleocapsid Protein of SARS-associated Coronavirus Upregulates the Transcription of hfgl2 Prothrombinase /fibroleukin Gene 1 2 1 2 1 1** YAO Hua-ning , WANG Zhan-hui , HAN Mei-fang , HOU Jin-lin , LUO Xiao-ping , NING Qin (1.Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2.Department of Infectious Diseases, Nanfang Hospital, Guangzhou 510515, China) Abstract ：The structural protein of SARS-associated coronavirus (SARS-CoV) responsible for the activation of hfgl2 gene was investigated. Gene fragments encoding the nucleocapsid, the membrane, and spike proteins were amplified by RT-PCR, and they were cloned into the eukaryotic expression vector pcDNA3.1 （+ ）. The plasmids were verified by restriction endonuclease analysis and sequencing. Chinese hamster ovary (CHO) cells were co-transfected with a plasmid carrying the hfgl2 promoter/luciferase and with one of the recombinant plasmids containing the genes enconding the N, M and S structural proteins. Luciferase activity was assayed as a measure of promoter function. Immunohistochemistry showed that genes encoding the structural protein of SARS-CoV were