Ezrin在肝癌中的表达及其与肿瘤黏附_侵袭和门静脉癌栓的关系_孙琦蛮.pdfVIP

( ) Fudan Univ J Med Sci 2005 Mar,32( 2) 147 Ezrin 孙琦蛮 樊 嘉 刘银坤 周 俭 余 耀 邱双健 吴志全 ( 200032) = (ezrin) 41 2 9 , RT-PCR ezrin MHCC97LMHCC97H LM3 3 ezrin ; MHCC97H ezrin , ezrin ( P 0.05) , ezrin ( P 0. 05) MHCC97LMHCC97H LM3 3 ezrin, MHCC97H ezrin Ezrin MHCC97LMHCC97HLM3 , ;Ezrin , = ; ; ; ; = R 735.7 EzrinExpressionof Hepatocellular Carcinoma andIts Relationship withAdhesion,InvasionandThrombosis in Portal Veinof Tumor SUN -i man,FAN Jia, LIU Yin-kun,ZHOU Jian, YU Yao, IU Shuang-jian,WU Zhi-quan ( L iv er Cancer Institut of Zhongshan Hosp ital, Fudan Univ ersity, Shanghai 200032, China) =Abstract Purpose To investigate membrane cytoskeleton crosslinker protein ezrin expression in hepato- cellular carcinoma(HCC) and itsrelationship with adhesion, invasion and portal vein thrombosisof tumor. ethods Forty-one HCC specimensand 9 benign specimen were involved in this study.The 41 HCC speci- menswere divided into high invasive ability group and low invasive ability group according to tumor size, daughter nodule, distant metastasis, envelope and portal vein tumor thrombosis.Ezrin was detected by RT- PCR and immunohistochemistry in these resection specimens and three HCC cell lines: MHCC97L MHCC97H and LM3.Antisense oligonucleotide of ezrin was transfected by liposomes into MHCC97H and its adhesive and invasive ability was compared with normal MHCC97H cells. Results Higher levels of ezrin mRNA and protein were showed in the HCC with high invasive ability than in those with low invasive ability(P 0.05) ,with the h