变形链球菌表面蛋白遗传多态性与粘附性能关系的研究.pdfVIP

_______________________________________________________________________________ 320临床医学 变形链球菌表面蛋白遗传多态性与粘附性能关系的研究 庄 姮，刘天佳，杨德琴，李 颂 （四川大学华西口腔医学院，四川省成都市 610041） [摘要] 目的 了解高、低粘附力变形链球菌临床分离株表面蛋白各粘附功能区遗传多 态性与其粘附性能的关系。 方法 实验菌株选自本实验室前期工作所获得的粘附力较强和 较弱的血清 c 型变形链球菌临床分离株，提取全菌 DNA，经PCR 分别扩增表面蛋白 A 区， P、V 区及C末端编码基因spaP-a 、spaP-pv 、spaP-c 后，用限制性内切酶HaeⅢ、AluI 进行 限制性片段长度多态性分析。19 株菌株进行 spaP-pv 的序列测定。 结果 1. 高、低粘附力 变链菌临床株扩增产物 spaP-a 经HaeⅢ酶切后，出现了 4 种基因型，其分布无统计学差异。 2. spaP-pv 经 AluI 酶切后，出现的 a 、b 2 种基因型菌株在不同粘附力菌株的分布不同 P0.05）。测序发现，a 型有 2 个片段与 b 型出现差异，并且均位于 V 区。3. spaP-c 经 （ AluI 酶切后呈现 2 种基因型，其分布无统计学差异。 结论 表面蛋白 V 区编码基因出现变 异可能是导致变链菌临床株粘附性能出现差异的原因之一。 [关键词] 变形链球菌; 表面蛋白; 遗传多态性; 粘附力 A study on the Relationship between genetic diversity within surface protein of Streptococcus mutans and Adherent abilities Zhuang Heng, Liu Tianjia, Yang Deqin, Li Song,. West China College of Stomatology, Sichuan University. Sichuan, Chengdu, 610041, China. [Abstract] Objective To study the relationship between genetic diversity within surface protein of Streptococcus mutans(serotype c) and their adherent abilities. Methods The clinical isolates of S.mutans included two groups with different adherent abilities, which were derived from previous work in our experimental lab. The bacterial DNA was extracted, and spaP-a 、 spaP-pv 、spaP-c were amplified respectively by PCR. Genetic diversity were assessed by restriction fragment-length polymorphism(RFLP) with restriction endonucleases HaeⅢ and AluI. Sequence of spaP-pv in 19 strains was performed. Results 1. Four different patterns of spaP-a PCR-RFLPs and two genotypes of spaP-pv we