利用脂肪酸生物标记（PLFA）分析猪舍基质垫层微生物亚群.docVIP

利用脂肪酸生物标记（PLFA）分析猪舍基质垫微生物亚群落分化 郑雪芳，刘波(，林营志 福建省农业科学院农业生物资源研究所，福州 350003 摘要猪舍基质垫层微生物群落不同时间猪舍基质垫层中的分布差异显著7:0 3OH仅分布于使用时间为1个月的基质垫层的第1、2、3层中，而使用时间为1个月的基质垫层的第4层以及使用时间为6、24个月的各层分布量极低。对猪舍基质垫层中的可2类群；欧氏距离为71.11时，可分为个可脂肪酸生物标记分为猪舍；基质垫层；磷脂脂肪酸（PLFA）Using PLFA biomarkers for analysis of the microbial subcommunity differentiation in the stroma cushion of pigsty ZHENG Xuefang, LIU Bo*, LIN Yingzhi, LAN Jianglin, LIU Danying Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fujian Fuzhou 350003 Received 6 February 2009; received in revised form …; accepted … Abstract: The PLFA biomarkers were introduced into analyzing the diversity of PLFA biomarkers for the microbial subcommunity in the stroma cushion of pigsty, which were treated by the microbial strains LUODON for one month, six months and twenty-four months，respectively. The results showed that PLFA biomarkers distributions were significant difference among the three different treating months in different layers with 20, 40, 60, 80 cm depth of the stroma cushion in the non-pollution pigsty. Some PLFA biomarkers distributed in all layers of different treating months in the stroma cushion, so called completely distribution. However, some PLFA biomarkers only distributed in the limited layers of stroma cushion, so called incompletely distribution. In addition, the distribution of individual PLFA biomarker was also remarkable different in the layers of the stroma cushions in each treating month, for example, the biomarker i17:0 3OH only distributed on the layer 1, layer 2 and layer 3, had a little distribution on the layer 4 of one month stroma cushion, that on the whole layers of six months and twenty-four months stroma cushions. In the cluster analysis, when mahalanobis distance was 56.62, the PLFA biomarkers in the stroma cushion of pigsty were grouped into three microbial subcommunities, named initial subcommunity, transitional subcommunity and stable subcommunity. The analysis of the chara