生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化.docVIP

生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化 曾庆才1，2，肖荣凤2，刘 波2*，胡桂萍2 福州大学生物科学与工程学院，福建 福州 350108；2.福建省农业科学院农业生物资源研究所，福建 福州 350003） 摘要：通过单因素，Box-Behnken 设计和响应面分析法优化生防菌哈茨木霉FJAT-9040固体发酵条件。以微生物发酵床养猪技术带来的新兴农业副产物——微生物养猪垫料为主要培养基质，以产孢量为指标，单因素实验确定初始含水量、初始接种量及培养温度分别为50.00%、5.00%、28.00℃。响应面分析结果表明：初始含水量和温度对产孢有显著相关性（p0.05），通过求解回归方程可得，当初始含水量为53.68%，初始接种量为7.00%，温度29.79℃，孢子含量达到2.02×1010个/g，与实际值1.43×1010个/g相近。 关键词：哈茨木霉；固体发酵；培养条件优化 Optimization of solid-state fermentation condition for the biocontrol strain of Trichoderma harzianum FJAT-9040 by response surface methodology Zeng Qing-cai 1,2 Xiao Rong-feng 2 Liu BO 2 Hu Gui-ping 2 College of Biological Science and Technology FuZhou University FuZhou FuJian 350108 China, 2. Agriculture Bio-resource Research Institute, FuJian Academy of Agriculture Science, FuZhou,FuJian 350003 China) Abstract: Optimization of solid-state fermentation condition for the biocontrol strain of Trichoderma harzianum FJAT-9040 using single factor analysis, Box-Behnken design.and response surface methodology (RSM). The medium was mainly bedding of microbial pig raising, which was from technology of piggery of microbial fermentation, and the sporulation yield was used as the indicator in all tests. The constitute of fermentation condition initial moisture, initial inoculation amount, culture temperature were determined by single factor experiment, then the optimal medium was optimized using Box-Behnken design and RSM. The result of single factor showed that the initial moisture, initial inoculation amount, culture temperature were 50.00%, 5.00%, 28℃, respectively. The results showed that initial moisture and culture temperature were significant for the spore of Trichoderma harzianum based on the response surface methodology analysis(P0.05).The optimum fermentation conditions were as followed: initial moisture 53.68%, initial inoculation amount 7.00%, culture temperature 28℃, the average yield of spore reached to 2.02×1010cfu/ g ,which was closed to the actual 1.43×1010 cfu/