内毒素血症所致脑血管痉挛与体液及神经内皮素_1表达的关系.pdfVIP

·562 · CHINESE JOURNAL OF CLINICAL ANATOMY VOL. 28 NO.5 2010 ·实验研究· 内毒素血症所致脑血管痉挛与体液及 神经内皮素-1 表达的关系 万丽丹，林雪群 南昌大学医学院人体解剖学教研室，南昌 330006 【摘要】 目的 探讨内毒素血症大鼠内皮素-1 与脑血管痉挛发生与发展的关系。 方法 96 只 Wistar 大鼠随机分为对照组、内毒素血症组(注射内毒素第3、6、12、24、48 h) ，以放射免疫检测血浆内皮素 -1 水平的变化，免疫组织化学ABC 法对大鼠大脑中动脉内皮素-1 能神经纤维进行染色观察。 结果 内毒素血症后3、6、12 h 大鼠血浆内皮素-1 水平较对照组明显升高(P0.05) ，内毒素血症后24 和48 h 已 趋于正常(P0.05) 。对照组和内毒素血症大鼠大脑中动脉可见棕褐色、呈细线状内皮素-1 能免疫反应阳 性纤维，以内毒素血症后第6、12 h 大鼠大脑中动脉内皮素-1 免疫反应最为显著(P0.05）。 结论 内毒 素血症后，大鼠内皮素-1 合成和释放入血水平升高，脑血管神经纤维内皮素-1 的表达水平上调，这两种 因素可能同时参与了脑血管痉挛的发生与发展。 【关键词】内毒素血症；脑血管痉挛；大脑中动脉；内皮素-1 【中图分类号】R331.36 【文献标识码】A 【文章编号】1001-165X（2010）05-562-04 The expression and distribution of endothelin-1 in middle cerebral arteries of endotoxemia rats WAN Li-dan, LIN Xue-qun Department of Anatomy, Medical School of Nanchang University, Nanchang 330006, China 【Abstract 】 Obj ective To investigate the relationship between endothelin-1 (ET-1) and cerebral vasospasm in endotoxemia rats. Methods 96 Wistar rats were randomly divided into control and experimental groups, the latter was then subdivided into 3, 6, 12, 24 and 48-h groups after the injection of lipopolysaccharide (LPS). The alteration of plasma ET-1 was detected by radioimmunoassay technique, and ET-1 immunoreactive nerve fibers in middle cerebral artery were examined with immunohistochemistry and measured with stereoscopy method. Results LPS treatment caused a significant increase of plasma ET-1 concentration at 3, 6 and 12 h after the treatment compared to that of control (P0.05). For 24 and 48-h LPS-treated groups, the plasma ET-1 decreased and closed to the normal level (P0.05). The ET-1 immunoreactive nerve fibers were ob