DNA芯片检测分枝杆菌的临床应用价值.pdfVIP

中华临床医师杂志( 电子版)2013 年12 月第7 卷第23 期 Chin J Clinicians(Electronic Edition),December 1,2013,Vol.7,No.23 ·10611 · ·临床论著· DNA 芯片检测分枝杆菌的临床应用价值 何莉 任微 梁雪妮 刘蒙蒙 孙炳奇 孟冬娅 【摘要】目的 比较DNA-微阵列芯片法和改良罗氏法检测结核分枝杆菌和非结核分枝杆菌的一致性， 探讨DNA-微阵列芯片法在临床上的应用价值。方法 应用DNA-微阵列芯片法检测64 例改良罗氏法阳性 的分枝杆菌核酸，比较两种方法检测结果的一致率；两种方法检测结果不一致样本进行测序验证，以测序 结果作为金标准，分析两种方法检测结果的正确率及不一致的可能原因。结果 两种方法检测总一致率为 93.8%，结核分枝杆菌检测一致率为87.5%，非结核分枝杆菌检测一致率为95.8% 。DNA-微阵列芯片法和改 良罗氏法共有4 例检测结果不一致，与测序结果比对后，改良罗氏法区分结核和非结核的正确率为96.9%； DNA-微阵列芯片法正确率为 93.8%，且能鉴定出7 种常见分枝杆菌。结论 DNA-微阵列芯片法与作为传 统金标准的改良罗氏法检测结核分枝杆菌和非结核分枝杆菌的一致性好、正确率高。操作简单、快速、灵 敏、并且能鉴定出常见分枝杆菌，DNA-微阵列芯片法可作为临床分枝杆菌感染诊断的重要检测手段。 【关键词】 分枝杆菌属； 基因芯片； 罗氏培养 Clinical value of gene chip detection of mycobacterium HE Li, REN Wei, LIANG Xue-ni, LIU Meng-meng, SUN Bing-qi, MENG Dong-ya. Department of Clinical Laboratory, General Hospital of Shenyang Military Region, Shenyang 110016, China Corresponding author: MENG Dong-ya, Email: mengdongya@ 【Abstract 】 Objective To compare the consistency of mycobacterium tuberculosis and non-tuberculous mycobacteria detected by Gene chip and Lowenstein-Jensen, then explore the value Gene chip clinical application. Methods 64 cases of Lowenstein-Jensen positive mycobacterial nucleic acid were detected by Gene chip to compare and analyse the consistency rate of two results and the possible reasons of incongruity results which would be verified by sequencing which is believed as the gold standard. Results The total concordance rate total concordance rate of two detecting methods was 93.8%, mycobacterium tuberculosis concordance rate was 87.5%, non-tuberculous mycobacteria was 95.8%. Comparing 4 cases of incongruity with sequencing results, Lowenstein - Jensen precision rate was 96.9% and Gene chip which can identify seven kinds of common mycobacterium was 93.8% in dist