临床研究大肠癌细胞中Oct4CD133的表达及意义.pdfVIP

·1434 · 南方医科大学学报（J South Med Univ ） 2011;31(8) ·临床研究· 大肠癌细胞中Oct4 与CD133的表达及意义 陈文晟，马 磊，胡晓晔，刘晓燕，金 川，李志彪，李卫东 广州医学院附属肿瘤医院，肿瘤临床研究中心，广东 广州 510095 摘要：目的探讨大肠癌细胞中转录因子Oct4 及干细胞标志物CD133抗原的表达情况及相关意义。方法采取术后大肠癌 石蜡包埋的组织标本30 例，应用免疫组化的方法分别检测癌组织及癌灶外邻近正常组织中Oct4 及CD133抗原的表达情 况。结果其中83.3% （25/30）的癌细胞标本中检测到Oct4 + 细胞，73.3% （22/30）检测到CD133+ 细胞。而且标本中 CD133+细胞的比例与Oct4+细胞的比例呈正相关（r=0.586 ，P0.05）。癌灶外邻近正常组织中检测到CD133+细胞与 Oct4+细胞均显著少于癌组织（P0.01）。结论大肠癌细胞中均有Oct4 与CD133 的表达，两者呈现正相关性，提示两者间 可能存在相互调节的机制，其关系及相关意义有待进一步研究。 关键词：大肠癌；Oct4 ；CD133 中图分类号：R735.3+4 文献标志码：A 文章编号：1673-4254（2011）08-1434-03 Expressions of OCT4 and CD133 and their correlation in colonic cancer CHEN Wen-sheng, MA Lei, HU Xiao-ye, LIU Xiao-yan, JIN Chuan, LI Zhi-biao, LI Wei-dong Institute of Cancer Research, Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou 510095, China Abstract: Objective To investigate the expressions of Oct4 and CD133 and their correlation in colonic cancer. Methods The expression of Oct4 and CD133 were detected by immunohistochemistry in 30 colon cancer specimens and the paired adjacent tissues. Results The positivity rates of Oct4 and CD133 expression were 83.3% (25/30) and 73.3% (22/ 30) in colonic cancer tissue, respectively, and their expressions were positively correlated (r=0.586, P0.05). The matched adjacent tissues showed significantly lower levels of Oct4 and CD133 expressions (P0.01). Conclusion The expressions of Oct4 and CD133 are upregulated in colonic cancer compared with those in the adjacent tissues and show a positive correlation. Oct4 and CD133 may play an important role in the development of colon cancer. Key words: colon cancer; Oct4; CD133 近年来很多相关的研究表明，结肠癌等上皮来源的 II-III 期。病理类型包括低分化腺癌14/30（