应用组织芯片技术探讨HBME_1_RET在良_恶性甲状腺病变中表达的意义.pdfVIP

·48 · J Diag Pathol ,February 2007 ,Vol. 14 ,No. 1 应用组织芯片技术探讨 HBME1 、RET 在良、恶性 甲状腺病变中表达的意义 孙 　洁 ,王翠芳 ,滕 　猛 ,宦大为 (沈阳医学院 奉天医院病理科 , 沈阳 　110024) [摘要] 　目的 　研究 HBME1 、RET 在良、恶性甲状腺病变中表达的特点 ,寻找有助于诊断良、恶性甲状腺病变的标 记物。方法 　应用组织芯片和免疫组化法检测 22 例结节性甲状腺肿、7 例甲状腺滤泡性腺瘤、47 例甲状腺乳头状 癌、7 例未分化癌、5 例髓样癌、4 例滤泡性癌、1 例低分化岛状癌及 1 例鳞状细胞癌中单克隆抗体 HBME1 、RET 的表 达。结果 　HBME1 、RET 在甲状腺癌中的表达阳性率分别为 6769 %和 2769 % ,在甲状腺良性病变中的表达阳性 率分别为 1034 %和 0 。结论 　HBME1 、RET 在甲状腺癌中的表达增加对明确诊断有一定价值。 [ 关键词] 　甲状腺; 　癌 ; 　HBME1 ; 　RET ( ) [ 中图分类号] 　R7361 　[文献标识码] 　A 　[文章编号]1007 - 8096 2007 01 - 0048 - 04 Expression of HBME-1 and RET and pathological changes in benign and malignant lesions of thyroids SUN Jie , WANG Cui-fnɡ, TENG Menɡ, et al . (Department of Pathology , Fenɡtiɑn Hospital , ShenyɑnɡMedical College , Shenyɑnɡ　110024 , China) Abstract : 　Objective 　To study the expression of HBME-1 and RET in benign and malignant pathological changes of thyroid , looking for valuable diagnostic markers to distringuish the beign lesions from the malignant ones. 　Methods 　By means of tissue microarray technique and immunohistochemical method , the expression of HBME-1 and RET was detected in 22 cases of nodular goiters , 7 cases of thyroid adenomas , 47 cases of papillary carcinomas , 7 cases of undifferentiated carcinoma , 5 cases of medullary thyroid carcinoma , 4 cases of follicular thyroid carcinomas , 1 case of poorly differentiated insular carcinoma and 1 case of squamous thyroid carcinoma. 　Results 　The positive rates of HBME-1 and RET were 6769 % and 2769 % in thyroid carcinomas , the ones of HBME-1 and RET were 1034 % and 0 in throid benign lesions , respectively. 　Conclusion 　The positive rates of HBEM-1 and RET are increas