反应时间对DPPH_法_ABTS_法评价抗氧化性结果的影响_林恋竹.pdfVIP

※基础研究 食品科学 2010, Vol. 31, No. 05 63 反应时间对DPPH ·法、ABTS+ ·法评价 抗氧化性结果的影响 林恋竹，赵谋明* (华南理工大学轻工与食品学院，广东广州 5 10640) 摘 要：由于D P P H ·法、A B T S+ ·法操作简单，不需要特殊的检测设备(通过反应体系颜色的改变评价试样抗 氧化性) ，因此，常被选作抗氧化性能评价方法。用这两种方法进行抗氧化性评价时，通常用固定反应时间下算 得的E C5 0 值( 自由基清除率为50 % 时所需试样浓度) 表征抗氧化剂的抗氧化性强弱(对于D P P H ·法，固定时间为 3 0m in ；对于A B T S+ ·法，固定时间为6m in ) ，结果表明：不同抗氧化剂因其组成、结构的差异，与D P P H ·、 AB T S+ ·反应速率不同，反应到达平衡的时间不同，将反应时间固定在某一值时，可能对抗氧化剂的抗氧化性评 价带来错误的判断。 关键词：反应时间；D P P H ·法；A B T S + ·法；抗氧化性评价 + Effect of Reaction Time on DPPH and ABTS Radical Scavenging Assays for Antioxidant Capacity Evaluation LIN Lian-zhu ，ZHAO Mou-ming* (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract ：Due to the operation simplicity of DPPH and ABTS+ radical scavenging arrays for evaluating antioxidant capacity and without a need for specific equipments, both assays have been widely chosen to evaluate antioxidant capacity. EC50 is generally used as a representative parameter for antioxidant capacity of antioxidants determined using DPPH ·assay at 30 min of fixed + reaction time or using ABTS ·assay at 6 min of fixed reaction time. The results of this investigation indicated that a big difference was made by the compositions and structures of antioxidants to reaction rates with DPPH ·and ABTS+ ·free radicals. The reaction equilibrium time was also different so that wrong estimations might