_电子_cDNA文库筛选指导基因的全长cDNA克隆.pdfVIP

生 物 技 术 通 讯 LETTERS IN BIOTECHNOLOGY　Vol . 11 　No . 1 　Mar . 2000 ·1 · 研究报告 “电子”cDNA 文库筛选指导基因的全长 cDNA 克隆 李 　刚　胡迎春 　张开泰 　吴德昌 (军事医学科学院放射医学研究所 　北京 　100850) ( ) 摘要 　“电子”cDNA 文库筛选主要是指通过采用生物信息学的方法延伸表达序列标签 EST 序列 , 以获得基因的部分乃至全长 cDNA 序列 ,避免或部分避免构建与筛选 cDNA 文库等烦琐的实验室工 作 。该方法具体体现了 EST 数据库的迅速扩张已导致识别与克隆新基因的策略发生革命性的变 化 。EST 序列 ZA73 为本实验室克隆到的可能参与辐射致气管上皮细胞恶性转化过程的基因片段 , 本研究采用“电子”cDNA 文库筛选的方法对其可能的全基因序列作了预测 。 关键词 　“电子”cDNA 文库筛选 ;表达序列标签 ; 电离辐射 ;基因 Electronic cD NA library screening directs the f ulllength cD NA cloning of a gene Li Gang , Hu Yingchun , Zhang Kaitai , Wu Dechang ( ) L aboratory of Molecular Toxicology , Institute of Radiation Medicins , B eij ing 100850 Abstract 　Electronic cDNA library screening mainly refers to extending ESTs sequence to get much of a gene using bioinformatics methods , bypassing much of the labwork of making and screening library . The emerging of electronic cDNA library screening indicates that the remarkable expansion of EST efforts has led to a revolu tionary change in the strategies for identifying and cloning novel genes. ZA73 is a gene fragment cloned by our laboratory which may be involved the neoplastic transformation of rat tracheal epithelial cells induced by radia tion . In this study , the potential nearly full gene sequence of ZA73 was predicted using electronic cDNA li brary screening method . Key words 　electronic cDNA library screening ; expressed sequence tags ; ion radiation ; gene 　　在通过 mR