T-DNA突变体研究多胚基因OsPE [Funct Integr Genomics].pdfVIP

Funct Integr Genomics DOI 10.1007/s10142-009-0139-6 ORIGINAL PAPER The polyembryo gene (OsPE) in rice Anju Puri P. Osman Basha Mankesh Kumar Deepak Rajpurohit Gursharn S. Randhawa Shahryar F. Kianian Anantharama Rishi Harcharan S. Dhaliwal Received: 5 May 2009 /Revised: 7 September 2009 /Accepted: 11 September 2009 # Springer-Verlag 2009 Abstract T-DNA insertional mutagenesis is one of the Keywords Oryza sativa . T-DNA/Ds . most important approaches for gene discovery and cloning. Insertional mutagenesis . Polyembryo . Cloning . T-DNA A fertile polyembryo mutant generated by T-DNA/Ds insertion in Oryza sativa, cv. Basmati 370 showed twin or triple seedlings at a frequency of 15–20%. T-DNA insertion Introduction was confirmed by 950 bp hpt gene amplification in the promoter region of the candidate gene. The annotated Rice, being one of the most important cereal crops, has protein corresponding to the OsPE candidate gene has been attracted major attention in functional genomics. It has reported as a hypothetical protein in O. sativa. OsPE gene become a model for monocot plants because of its relatively lacked functional homologs in other species. No OsPE small genome size among cereals (389 Mb); ease of paralog was found in rice. No conserved domains were transformation; availability of detailed physical and dense found in the protein coded by OsPE. RT-PCR showed the molecular marker maps, existence of collinearity and expression of OsPE gene in Basmati 370 shoots. Full- synteny in gene sequence and gene function among all length OsPE gene was cloned in Basmati 370. The cereals and grasses, as well as its economic importan