High-resolutionexvivoimaginginmousespinalcordusingmicro教程.pdfVIP

Neuroscience Research 73 (2012) 337–340 Contents lists available at SciVerse ScienceDirect Neuroscience Research j o u r n a l h o m e p a g e: w w w. elsev /locate/neures Technical note High-resolution ex vivo imaging in mouse spinal cord using micro-CT with 11.7T-MRI and myelin staining validation Shigeyoshi Saito a,∗ , Yuki Mori b , Yoshichika Yoshioka b , Kenya Murase a a Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, 1-7Yamadaoka, Suita, Osaka 565-0871, Japan b High Performance Bioimaging Research Facility, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Annex 1F, Suita, Osaka 565-0871, Japan a r t i c l e i n f o a b s t r a c t Article history: We investigated the use of micro-CT with contrast agent for the characterization of fixed mouse spinal Received 10 February 2012 cord as a means to differentiate between gray and white matter. The spinal cords were soaked in a con- Received in revised form 6 May 2012 centration of nonionic iodinated contrast agent for 14 days. Micro-CT was performed and then compared Accepted 8 May 2012 using 11.7T-MRI images and myelin staining. Soaking the spinal cords in contrast agent resulted in clear Available online 16 May 2012 differences in signal between the gray and white matter at 3 planes. Micro-CT provides more relevant information on mouse spinal cord GM and W